Biosensors and Bioelectronics, volume 171, pages 112679
Multiplexed detection and quantification of human antibody response to COVID-19 infection using a plasmon enhanced biosensor platform
Nathaniel Cady
1
,
Natalya Tokranova
1
,
Armond Minor
1
,
Nima Nikvand
1
,
Klemen Strle
2
,
William M. Lee
2
,
William Page
3
,
ERNEST GUIGNON
3
,
Arturo Pilar
3
,
George B. Gibson
4, 5
1
College of Nanoscale Science & Engineering, SUNY Polytechnic Institute, Albany, NY, USA.
|
2
Wadsworth Center, New York State Department of Health, Albany, NY, USA and School of Public Health, University at Albany, Albany, NY, USA
|
3
Ciencia, Inc., East Hartford, CT, USA.
|
4
Ciencia, Inc., East Hartford, CT, USA
|
Publication type: Journal Article
Publication date: 2021-01-01
Journal:
Biosensors and Bioelectronics
scimago Q1
wos Q1
SJR: 2.052
CiteScore: 20.8
Impact factor: 10.7
ISSN: 09565663, 18734235
General Medicine
Biophysics
Electrochemistry
Biotechnology
Biomedical Engineering
Abstract
The 2019 SARS CoV-2 (COVID-19) pandemic has illustrated the need for rapid and accurate diagnostic tests. In this work, a multiplexed grating-coupled fluorescent plasmonics (GC-FP) biosensor platform was used to rapidly and accurately measure antibodies against COVID-19 in human blood serum and dried blood spot samples. The GC-FP platform measures antibody-antigen binding interactions for multiple targets in a single sample, and has 100% selectivity and sensitivity (n = 23) when measuring serum IgG levels against three COVID-19 antigens (spike S1, spike S1S2, and the nucleocapsid protein). The GC-FP platform yielded a quantitative, linear response for serum samples diluted to as low as 1:1600 dilution. Test results were highly correlated with two commercial COVID-19 antibody tests, including an enzyme linked immunosorbent assay (ELISA) and a Luminex-based microsphere immunoassay. To demonstrate test efficacy with other sample matrices, dried blood spot samples (n = 63) were obtained and evaluated with GC-FP, yielding 100% selectivity and 86.7% sensitivity for diagnosing prior COVID-19 infection. The test was also evaluated for detection of multiple immunoglobulin isotypes, with successful detection of IgM, IgG and IgA antibody-antigen interactions. Last, a machine learning approach was developed to accurately score patient samples for prior COVID-19 infection, using antibody binding data for all three COVID-19 antigens used in the test. • GC-FP biosensor yields rapid (30 min) detection of antibodies against COVID-19 infection. • Antibody detection is quantitative and can provide antibody titer information. • Diagnosis of prior COVID-19 infection using serum with 100% selectivity and sensitivity. • Multiplexed approach yields information on multiple antibody-antigen interactions. • Dried blood spot testing significantly simplifies sample collection and yields high sensitivity/specificity.
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Cady N. et al. Multiplexed detection and quantification of human antibody response to COVID-19 infection using a plasmon enhanced biosensor platform // Biosensors and Bioelectronics. 2021. Vol. 171. p. 112679.
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Cady N., Tokranova N., Minor A., Nikvand N., Strle K., Lee W. M., Page W., GUIGNON E., Pilar A., Gibson G. B. Multiplexed detection and quantification of human antibody response to COVID-19 infection using a plasmon enhanced biosensor platform // Biosensors and Bioelectronics. 2021. Vol. 171. p. 112679.
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TY - JOUR
DO - 10.1016/j.bios.2020.112679
UR - https://doi.org/10.1016/j.bios.2020.112679
TI - Multiplexed detection and quantification of human antibody response to COVID-19 infection using a plasmon enhanced biosensor platform
T2 - Biosensors and Bioelectronics
AU - Cady, Nathaniel
AU - Tokranova, Natalya
AU - Minor, Armond
AU - Nikvand, Nima
AU - Strle, Klemen
AU - Lee, William M.
AU - Page, William
AU - GUIGNON, ERNEST
AU - Pilar, Arturo
AU - Gibson, George B.
PY - 2021
DA - 2021/01/01
PB - Elsevier
SP - 112679
VL - 171
SN - 0956-5663
SN - 1873-4235
ER -
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@article{2021_Cady,
author = {Nathaniel Cady and Natalya Tokranova and Armond Minor and Nima Nikvand and Klemen Strle and William M. Lee and William Page and ERNEST GUIGNON and Arturo Pilar and George B. Gibson},
title = {Multiplexed detection and quantification of human antibody response to COVID-19 infection using a plasmon enhanced biosensor platform},
journal = {Biosensors and Bioelectronics},
year = {2021},
volume = {171},
publisher = {Elsevier},
month = {jan},
url = {https://doi.org/10.1016/j.bios.2020.112679},
pages = {112679},
doi = {10.1016/j.bios.2020.112679}
}