Developmental and Comparative Immunology, volume 122, pages 104126

RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection.

Grizanova Ekaterina V ¹

Coates Christopher ²

BUTT T. M. ²

Dubovskiy Ivan M. ^{3, 4}

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Novosibirsk State Agrarian University, Laboratory of Biological Plant Protection and Biotechnology, Dobrolubova str. 160, 630039 Novosibirsk, Russia. |

Department of Biosciences, Faculty of Science and Engineering, Swansea University, Swansea, SA2 8PP, Wales, UK. |

Novosibirsk State Agrarian University, Laboratory of Biological Plant Protection and Biotechnology, Dobrolubova Str. 160, 630039, Novosibirsk, Russia |

⁴

Siberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of Sciences, Russia |

Publication type: Journal Article

Publication date: 2021-09-01

Elsevier

Journal: Developmental and Comparative Immunology

Quartile SCImago

Quartile WOS

Impact factor: 2.9

ISSN: 0145305X, 18790089

DOI: 10.1016/j.dci.2021.104126

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Developmental Biology

Immunology

Abstract

The co-evolutionary arms race between disease-causing agents and their insect victims is ancient and complex - leading to the development of specialised attack and defence strategies. Among such strategies is the capacity of fungal and oomycete pathogens to deploy degradative enzymes, notably proteases, to facilitate infection directly across the integument. To counter these proteases, insects such as the greater wax moth Galleria mellonella release metalloprotease inhibitors and other immune factors to thwart the invading fungus. To date, molecular-based confirmation of insect metalloprotease inhibitor's incontrovertible role in antifungal defence has been lacking. We targeted the IMPI gene for suppression using RNAi and exposed those insects to the entomopathogenic fungus Metarhizium brunneum ARSEF4556. Levels of IMPI were reduced significantly in the integument (10-fold) and fat body (5-fold) of RNAi-treated insects when compared to control larvae, and displayed a significantly higher mortality rate. We also surveyed candidate immune/detoxification gene expression levels (e.g., DOPA decarboxylase, galiomycin) in three tissues (integument, midgut, fat body) in order to gauge any potential non-target effects of RNAi. The loss of IMPI via RNAi compromises antifungal defences and leaves G. mellonella vulnerable to infection.

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Citations by journals

	1 2
Insects	Insects, 2, 20% Insects 2 publications, 20%
Nanomaterials	Nanomaterials, 1, 10% Nanomaterials 1 publication, 10%
Microbial Pathogenesis	Microbial Pathogenesis, 1, 10% Microbial Pathogenesis 1 publication, 10%
Archives of Toxicology	Archives of Toxicology, 1, 10% Archives of Toxicology 1 publication, 10%
Food and Chemical Toxicology	Food and Chemical Toxicology, 1, 10% Food and Chemical Toxicology 1 publication, 10%
Insect Science	Insect Science, 1, 10% Insect Science 1 publication, 10%
Journal of Fungi	Journal of Fungi, 1, 10% Journal of Fungi 1 publication, 10%
Frontiers in Immunology	Frontiers in Immunology, 1, 10% Frontiers in Immunology 1 publication, 10%
	1 2

Citations by publishers

	1 2 3 4
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 4, 40% Multidisciplinary Digital Publishing Institute (MDPI) 4 publications, 40%
Elsevier	Elsevier, 2, 20% Elsevier 2 publications, 20%
Springer Nature	Springer Nature, 2, 20% Springer Nature 2 publications, 20%
Wiley	Wiley, 1, 10% Wiley 1 publication, 10%
Frontiers Media S.A.	Frontiers Media S.A., 1, 10% Frontiers Media S.A. 1 publication, 10%
	1 2 3 4

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Grizanova E. V. et al. RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection. // Developmental and Comparative Immunology. 2021. Vol. 122. p. 104126.

GOST all authors (up to 50) Copy

Grizanova E. V., Coates C., BUTT T. M., Dubovskiy I. M. RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection. // Developmental and Comparative Immunology. 2021. Vol. 122. p. 104126.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1016/j.dci.2021.104126

UR - https://doi.org/10.1016%2Fj.dci.2021.104126

TI - RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection.

T2 - Developmental and Comparative Immunology

AU - Grizanova, Ekaterina V

AU - Coates, Christopher

AU - BUTT, T. M.

AU - Dubovskiy, Ivan M.

PY - 2021

DA - 2021/09/01 00:00:00

PB - Elsevier

SP - 104126

VL - 122

SN - 0145-305X

SN - 1879-0089

ER -

BibTex

Cite this

BibTex Copy

@article{2021_Grizanova,

author = {Ekaterina V Grizanova and Christopher Coates and T. M. BUTT and Ivan M. Dubovskiy},

title = {RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection.},

journal = {Developmental and Comparative Immunology},

year = {2021},

volume = {122},

publisher = {Elsevier},

month = {sep},

url = {https://doi.org/10.1016%2Fj.dci.2021.104126},

pages = {104126},

doi = {10.1016/j.dci.2021.104126}

}

Found error?

Publisher

Elsevier

Journal

Developmental and Comparative Immunology

Quartile SCImago

Quartile WOS

Impact factor

2.9

ISSN

0145305X (Print)
18790089 (Electronic)

Labs

Laboratory of Biological Plant Protection and Biotechnology

Profiles

Dubovskiy, Ivan M