Developmental Cell

, volume 58 , issue 16 , pages 1477-148800000

Loss of TJP1 disrupts gastrulation patterning and increases differentiation toward the germ cell lineage in human pluripotent stem cells

Ivana Vasic ^{1, 2}

Ashley R. G. Libby ^{1, 3}

Annie Maslan ^{2, 4, 5}

Emily A. Bulger ^{1, 3}

David Zalazar ¹

Martina Z. Krakora Compagno ¹

Aaron M. Streets ^{2, 4, 5, 6}

Kiichiro Tomoda ^{1, 7}

Shinya Yamanaka ^{1, 7}

Todd C. McDevitt ^{1, 8}

Hide authors affiliations Show authors affiliations: 8 affiliations

Gladstone Institute of Cardiovascular Disease, Gladstone Institutes, San Francisco, CA 94158, USA |

UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, San Francisco, San Francisco, CA 94158, USA

University of California, Berkeley

University of California, San Francisco

Developmental and Stem Cell Biology Ph.D. Program, University of California, San Francisco, San Francisco, CA 94158, USA |

⁴

Center for Computational Biology, University of California, Berkeley, Berkeley, CA 94720, USA |

⁵

Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA |

⁶

Chan Zuckerberg BioHub, San Francisco, CA 94158, USA |

⁷

Center for iPS Cell Research and Application, Kyoto 606-8397, Japan |

⁸

Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA 94158, USA |

Publication type: Journal Article

Publication date: 2023-08-01

Elsevier

Developmental Cell

scimago Q1

wos Q1

SJR: 5.180

CiteScore: 16.7

Impact factor: 8.7

ISSN: 15345807, 18781551

DOI: 10.1016/j.devcel.2023.05.019

Copy DOI

PubMed ID: 37354899

General Biochemistry, Genetics and Molecular Biology

Molecular Biology

Cell Biology

Developmental Biology

Abstract

Biological patterning events that occur early in development establish proper tissue morphogenesis. Identifying the mechanisms that guide these patterning events is necessary in order to understand the molecular drivers of development and disease and to build tissues in vitro. In this study, we use an in vitro model of gastrulation to study the role of tight junctions and apical/basolateral polarity in modulating bone morphogenic protein-4 (BMP4) signaling and gastrulation-associated patterning in colonies of human pluripotent stem cells (hPSCs). Disrupting tight junctions via knockdown (KD) of the scaffolding tight junction protein-1 (TJP1, also known as ZO1) allows BMP4 to robustly and ubiquitously activate pSMAD1/5 signaling over time, resulting in loss of the patterning phenotype and marked differentiation bias of pluripotent stem cells to primordial germ cell-like cells (PGCLCs). These findings give important insights into how signaling events are regulated and lead to spatial emergence of diverse cell types in vitro.

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Vasic I. et al. Loss of TJP1 disrupts gastrulation patterning and increases differentiation toward the germ cell lineage in human pluripotent stem cells // Developmental Cell. 2023. Vol. 58. No. 16. pp. 1477-148800000.

GOST all authors (up to 50) Copy

Vasic I., Libby A. R. G., Maslan A., Bulger E. A., Zalazar D., Krakora Compagno M. Z., Streets A. M., Tomoda K., Yamanaka S., McDevitt T. C. Loss of TJP1 disrupts gastrulation patterning and increases differentiation toward the germ cell lineage in human pluripotent stem cells // Developmental Cell. 2023. Vol. 58. No. 16. pp. 1477-148800000.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1016/j.devcel.2023.05.019

UR - https://doi.org/10.1016/j.devcel.2023.05.019

TI - Loss of TJP1 disrupts gastrulation patterning and increases differentiation toward the germ cell lineage in human pluripotent stem cells

T2 - Developmental Cell

AU - Vasic, Ivana

AU - Libby, Ashley R. G.

AU - Maslan, Annie

AU - Bulger, Emily A.

AU - Zalazar, David

AU - Krakora Compagno, Martina Z.

AU - Streets, Aaron M.

AU - Tomoda, Kiichiro

AU - Yamanaka, Shinya

AU - McDevitt, Todd C.

PY - 2023

DA - 2023/08/01

PB - Elsevier

SP - 1477-148800000

IS - 16

VL - 58

PMID - 37354899

SN - 1534-5807

SN - 1878-1551

ER -

BibTex |

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BibTex (up to 50 authors) Copy

@article{2023_Vasic,

author = {Ivana Vasic and Ashley R. G. Libby and Annie Maslan and Emily A. Bulger and David Zalazar and Martina Z. Krakora Compagno and Aaron M. Streets and Kiichiro Tomoda and Shinya Yamanaka and Todd C. McDevitt},

title = {Loss of TJP1 disrupts gastrulation patterning and increases differentiation toward the germ cell lineage in human pluripotent stem cells},

journal = {Developmental Cell},

year = {2023},

volume = {58},

publisher = {Elsevier},

month = {aug},

url = {https://doi.org/10.1016/j.devcel.2023.05.019},

number = {16},

pages = {1477--148800000},

doi = {10.1016/j.devcel.2023.05.019}

}

MLA

Cite this

MLA Copy

Vasic, Ivana, et al. “Loss of TJP1 disrupts gastrulation patterning and increases differentiation toward the germ cell lineage in human pluripotent stem cells.” Developmental Cell, vol. 58, no. 16, Aug. 2023, pp. 1477-148800000. https://doi.org/10.1016/j.devcel.2023.05.019.

Publisher

Elsevier

Journal

Developmental Cell

scimago Q1

wos Q1

SJR

5.180

CiteScore

16.7

Impact factor

8.7

ISSN

15345807 (Print)

18781551 (Electronic)