Evolution and recent development of cellulose-modified, nucleic acid-based and green nanosensors for trace heavy metal ion analyses in complex media: A review

With increased manufacturing activities and energy sector development, monitoring of heavy metal ion (HMI) pollution is becoming increasingly pressing. The discharge of metals from industrial effluents into the waterways could cause major economic and environmental disruption. In situ and on-site detection methods of trace HMIs can be effective countermeasures before the toxicity spreads out to larger areas, affecting the ecosystem. Conventional methods are often lacking in portability and costly. In contrast, electrochemical sensing, especially with nanoplatforms, is promising for trace detection of HMIs in complex media because of the ease of fabrication and adaptability of incorporating green technology. Appropriate electrode selection with suitable modifiers is crucial in complex medium analyses to overcome electrode fouling. In this review, the evolution from metal-based and carbon-based electrodes to advancements in electrode modification involving agro/biocomposite nanomaterials (NMs) such as cellulose, chitosan, and hydroxyapatite is discussed. The fabrication of nucleic acid-based aptasensors for analyzing HMIs and the adoption of smart systems based on microfluidics with high selectivity, operational stability, and sensitivity are highlighted. The challenges and future prospects for trace HMI determination based on electrochemical sensors in real complex media, including blood and industrial effluent or wastewater, are critically examined.