,
volume 843
,
issue 2
,
pages 164-169
High-performance liquid chromatographic method for the determination of pioglitazone in human plasma using ultraviolet detection and its application to a pharmacokinetic study
1
Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences
3
Phitsanulok 65000 Thailand
|
Publication type: Journal Article
Publication date: 2006-11-07
scimago Q2
wos Q2
SJR: 0.568
CiteScore: 5.4
Impact factor: 2.8
ISSN: 15700232, 1873376X
PubMed ID:
16815107
Biochemistry
General Medicine
Cell Biology
Clinical Biochemistry
Analytical Chemistry
Abstract
An analytical method based on high-performance liquid chromatography (HPLC) with ultraviolet detection (269 nm) was developed for the determination of pioglitazone in human plasma. Rosiglitazone was used as an internal standard. Chromatographic separation was achieved with a reversed-phase Apollo C18 column and a mobile phase of methanol-acetonitrile-mixed phosphate buffer (pH 2.6; 10mM) (40:12:48, v/v/v) with a flow rate of 1.2 ml/min. The calibration curve was linear over the range of 50-2000 ng/ml (r(2)>0.9987) and the lower limit of quantification was 50 ng/ml. The method was validated with excellent sensitivity, accuracy, precision, recovery and stability. The assay has been applied successfully to a pharmacokinetic study with human volunteers.
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Metrics
53
Total citations:
53
Citations from 2024:
4
(7.55%)
The most citing journal
Citations in journal:
7
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GOST
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Sripalakit P. et al. High-performance liquid chromatographic method for the determination of pioglitazone in human plasma using ultraviolet detection and its application to a pharmacokinetic study // Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2006. Vol. 843. No. 2. pp. 164-169.
GOST all authors (up to 50)
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Sripalakit P., Neamhom P., Saraphanchotiwitthaya A. High-performance liquid chromatographic method for the determination of pioglitazone in human plasma using ultraviolet detection and its application to a pharmacokinetic study // Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2006. Vol. 843. No. 2. pp. 164-169.
Cite this
RIS
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TY - JOUR
DO - 10.1016/j.jchromb.2006.05.032
UR - https://doi.org/10.1016/j.jchromb.2006.05.032
TI - High-performance liquid chromatographic method for the determination of pioglitazone in human plasma using ultraviolet detection and its application to a pharmacokinetic study
T2 - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
AU - Sripalakit, P
AU - Neamhom, P
AU - Saraphanchotiwitthaya, A.
PY - 2006
DA - 2006/11/07
PB - Elsevier
SP - 164-169
IS - 2
VL - 843
PMID - 16815107
SN - 1570-0232
SN - 1873-376X
ER -
Cite this
BibTex (up to 50 authors)
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@article{2006_Sripalakit,
author = {P Sripalakit and P Neamhom and A. Saraphanchotiwitthaya},
title = {High-performance liquid chromatographic method for the determination of pioglitazone in human plasma using ultraviolet detection and its application to a pharmacokinetic study},
journal = {Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences},
year = {2006},
volume = {843},
publisher = {Elsevier},
month = {nov},
url = {https://doi.org/10.1016/j.jchromb.2006.05.032},
number = {2},
pages = {164--169},
doi = {10.1016/j.jchromb.2006.05.032}
}
Cite this
MLA
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Sripalakit, P., et al. “High-performance liquid chromatographic method for the determination of pioglitazone in human plasma using ultraviolet detection and its application to a pharmacokinetic study.” Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 843, no. 2, Nov. 2006, pp. 164-169. https://doi.org/10.1016/j.jchromb.2006.05.032.