The transcription factor CsPAT1 from tea plant (Camellia sinensis) is involved in drought tolerance by modulating phenylpropanoid biosynthesis

Tea plants, in particular, leafy cash crops, prefer warm and humid climates. Our previous work identified CsPAT1 as a facilitator of lignin biosynthesis in tea plants. The specific role of CsPAT1 in tea plants’ abiotic stress response remains unclear. In this study, we found that the expression of CsPAT1 in tea plants was induced under drought, cold, heat, and ABA treatments. CsPAT1 transgenic Arabidopsis lines displayed enhanced drought tolerance compared with wild-type (WT) controls. The SOD and POD activities, proline content, and expression levels of drought-responsive genes were significantly increased in transgenic Arabidopsis under drought stress treatment. Transcriptome analysis revealed a significant enrichment of differentially expressed genes (DEGs) in the flavonoid biosynthesis pathway. Correspondingly, total flavonoid contents were significantly higher in the CsPAT1 transgenic lines. Through UPLC–MS/MS-based flavonoid metabolome analysis, we identified and quantified 24 flavonoid metabolites. Notably, CsPAT1 transgenic lines exhibited significantly lower levels of phenylpropanoids and hydroxycinnamic acids, key precursors in phenylpropanoid biosynthesis. Conversely, nine flavonoid compounds were significantly elevated in the transgenic lines, including apigenin, luteolin 7-O-glucoside, kaempferide, naringenin, butin, catechin, biochanin A, daidzin, and genistein. These findings suggest that CsPAT1 may enhance drought resistance by regulating the phenylpropanoid metabolic pathway. Our results provide insights for future breeding strategies to enhance drought tolerance in tea plants.