Microbial Pathogenesis, volume 175, pages 105958
Virulent and necrotrophic strategies of Bacillus thuringiensis in susceptible and resistant insects, Galleria mellonella
7
Zoology, Ryan Institute, School of Natural Sciences, University of Galway, Galway, H91 TK33, Ireland
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8
Department of Biosciences, Faculty of Science and Engineering, Swansea University, Swansea, SA2 8PP, Wales, UK. Electronic address: christopher.coates@nuigalway.ie.
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Publication type: Journal Article
Publication date: 2023-02-01
Journal:
Microbial Pathogenesis
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor: 3.8
ISSN: 08824010, 10961208
Microbiology
Infectious Diseases
Abstract
Bacillus thuringiensis (Bt) is one of the most common entomopathogenic bacteria used as a biopesticide, and source of endotoxin genes for generating insect-resistant transgenic plants. The mechanisms underpinning an insect's susceptibility or resistance to B. thuringiensis are diverse. The bacterial lifecycle does not end with the death of a host, they continue to exploit the cadaver to reproduce and sporulate. Herein, we studied the progression of B. thuringiensis subsp. galleriae infection in two populations of wax moth larvae (Galleria mellonella) to gain further insight into the "arms race" between B. thuringiensis virulence and insect defences. Two doses of B. thuringiensis subsp. galleriae (spore and crystalline toxin mixtures) were administered orally to compare the responses of susceptible (S) and resistant (R) populations at ∼30% mortality each. To investigate B. thuringiensis-insect antibiosis, we used a combination of in vivo infection trials, bacterial microbiome analysis, and RNAi targeting the antibacterial peptide gloverin. Within 48 h post-inoculation, B. thuringiensis-resistant insects purged the midgut of bacteria, i.e., colony forming unit numbers fell below detectable levels. Second, B. thuringiensis rapidly modulated gene expression to initiate sporulation (linked to quorum sensing) when exposed to resistant insects in contrast to susceptible G. mellonella. We reinforce earlier findings that elevated levels of antimicrobial peptides, specifically gloverin, are found in the midgut of resistant insects, which is an evolutionary strategy to combat B. thuringiensis infection via its main portal of entry. A sub-population of highly virulent B. thuringiensis can survive the enhanced immune defences of resistant G. mellonella by disrupting the midgut microbiome and switching rapidly to a necrotrophic strategy, prior to sporulation in the cadaver.
Citations by journals
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1
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Agronomy
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Agronomy
1 publication, 33.33%
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Advances in Insect Physiology
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Advances in Insect Physiology
1 publication, 33.33%
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1
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Citations by publishers
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1
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 33.33%
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Elsevier
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Elsevier
1 publication, 33.33%
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1
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Grizanova E. V. et al. Virulent and necrotrophic strategies of Bacillus thuringiensis in susceptible and resistant insects, Galleria mellonella // Microbial Pathogenesis. 2023. Vol. 175. p. 105958.
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Grizanova E. V., Krytsyna T. I., Kalmykova G. V., Sokolova E., Alikina T., Kabilov M. R., Coates C., Dubovskiy I. M. Virulent and necrotrophic strategies of Bacillus thuringiensis in susceptible and resistant insects, Galleria mellonella // Microbial Pathogenesis. 2023. Vol. 175. p. 105958.
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TY - JOUR
DO - 10.1016/j.micpath.2022.105958
UR - https://doi.org/10.1016%2Fj.micpath.2022.105958
TI - Virulent and necrotrophic strategies of Bacillus thuringiensis in susceptible and resistant insects, Galleria mellonella
T2 - Microbial Pathogenesis
AU - Grizanova, Ekaterina V
AU - Krytsyna, Tatiana I
AU - Kalmykova, Galina V
AU - Sokolova, Elina
AU - Alikina, Tatiana
AU - Kabilov, Marsel R.
AU - Coates, Christopher
AU - Dubovskiy, Ivan M.
PY - 2023
DA - 2023/02/01 00:00:00
PB - Elsevier
SP - 105958
VL - 175
SN - 0882-4010
SN - 1096-1208
ER -
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@article{2023_Grizanova,
author = {Ekaterina V Grizanova and Tatiana I Krytsyna and Galina V Kalmykova and Elina Sokolova and Tatiana Alikina and Marsel R. Kabilov and Christopher Coates and Ivan M. Dubovskiy},
title = {Virulent and necrotrophic strategies of Bacillus thuringiensis in susceptible and resistant insects, Galleria mellonella},
journal = {Microbial Pathogenesis},
year = {2023},
volume = {175},
publisher = {Elsevier},
month = {feb},
url = {https://doi.org/10.1016%2Fj.micpath.2022.105958},
pages = {105958},
doi = {10.1016/j.micpath.2022.105958}
}