An enantioselective stochastic miniplatform based on a sensor designed using poly-Allura Red, TiO2 and carbon nanopowder for enantioanalysis of valine in whole blood samples

In the search for new analytes that can be applied to the early identification of carcinoma, metabolomics becomes an important component. Therefore, the enantioanalysis of valine was done by screening of whole blood samples using an enantioselective stochastic miniplatform based on a sensor designed using poly-Allura Red, TiO2 and carbon nanopowder. The working concentration ranges registered for the L- and D- enantiomers were between 1 × 10−13 mol/L and 1 × 10−11 mol/L, and between 1 × 10−12 mol/L and 1 × 10−10 mol/L, respectively. Sensitivities of magnitude orders of 1010 and 109 s−1 mol−1 L were registered for the L- and D-valine. L- and D-valine were recovered in whole blood samples with recoveries exceeding 99.80 % (%, RSD less than 1.00 %) demonstrating that they may be consistently used for the enantioanalysis of valine in whole blood samples. Screening tests of whole blood from healthy volunteers and patients diagnosed with lung cancer shown that the D-valine was only found in the whole blood of the patients diagnosed with lung cancer.