Micron

, volume 163 , pages 103360

Spatio-temporal segmentation of image sequences for non-invasive analysis of cardiovascular structure and function in Whitefish embryos

Alexander Machikhin ^{1, 2}

A.V. Guryleva ^{1, 2}

A G Selyukov ³

A B Burlakov ^{1, 2, 4}

V.I. Bukova ^{1, 2, 5}

Demid D Khokhlov ^{1, 2}

E. V. EFREMOVA ⁶

E. E. Rudenko ⁷

Hide authors affiliations Show authors affiliations: 7 affiliations

15 Butlerova street, Moscow 117342, Russia |

Scientific and Technological Center of Unique Instrumentation of Russian Academy of Sciences |

Tyumen State University, 3 Pirogova street, Tyumen 625043, Russia |

⁴

Department of Ichthyology, Faculty of Biology, Lomonosov Moscow State University, 1–12, Leninskie Gory, Moscow 119991, Russia |

⁵

Bauman Moscow State Technical University, 5 2-ya Baumanskaya street, Moscow 105005, Russia |

⁶

Sob fish hatchery, Sob embankment, Labytnangi (Kharp urban-type settlement), 629420, Russia |

⁷

Sechenov First Moscow State Medical University, 2 Bolshaya Pirogovskaya street, Moscow 119991, Russia |

Publication type: Journal Article

Publication date: 2022-12-01

Elsevier

Micron

scimago Q2

wos Q1

SJR: 0.581

CiteScore: 5.3

Impact factor: 2.2

ISSN: 09684328, 18784291

DOI: 10.1016/j.micron.2022.103360

Copy DOI

PubMed ID: 36274447

Cell Biology

Structural Biology

General Physics and Astronomy

General Materials Science

Abstract

Label-free quantitative analysis of the cardiovascular structure and function in wild fish is highly important and still unsolved problem. We developed a robust algorithm for bright field microscopy data processing, which allows reliable detection and quantification of circulatory system across the whole animal. We verified this technique by in vivo experiments on broad whitefish (Coregonus nasus) embryos. Calculated morphofunctional features of heart and vessels are quite specific due to low temperature embryonic development of this fish, and correspond well to the reference data obtained by ex vivo techniques. This research highlights the feasibility of non-invasive cardiovascular imaging and measurements valuable for behavioral, environmental, toxicological, drug discovery and many other studies.

Found

6 citations

Gurileva Anastasiya

PhD in Engineering

33 publications, 68 citations

h-index: 5

Bauman Moscow State Technical University

Scientific and Technological Centre of Unique Instrumentation of the Russian Academy of Sciences

Research interests

Optics

Spectroscopy

2 citations

Shuman Leonid

9 publications, 21 citations

h-index: 3

University of Tyumen

Top-30

Journals

	1
Journal of Experimental Biology	Journal of Experimental Biology, 1, 14.29% Journal of Experimental Biology 1 publication, 14.29%
Journal of Biophotonics	Journal of Biophotonics, 1, 14.29% Journal of Biophotonics 1 publication, 14.29%
Antioxidants and Redox Signaling	Antioxidants and Redox Signaling, 1, 14.29% Antioxidants and Redox Signaling 1 publication, 14.29%
Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology	Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 1, 14.29% Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology 1 publication, 14.29%
IEEE Transactions on Instrumentation and Measurement	IEEE Transactions on Instrumentation and Measurement, 1, 14.29% IEEE Transactions on Instrumentation and Measurement 1 publication, 14.29%
	1

Publishers

	1 2
Institute of Electrical and Electronics Engineers (IEEE)	Institute of Electrical and Electronics Engineers (IEEE), 2, 28.57% Institute of Electrical and Electronics Engineers (IEEE) 2 publications, 28.57%
The Company of Biologists	The Company of Biologists, 1, 14.29% The Company of Biologists 1 publication, 14.29%
Wiley	Wiley, 1, 14.29% Wiley 1 publication, 14.29%
Mary Ann Liebert	Mary Ann Liebert, 1, 14.29% Mary Ann Liebert 1 publication, 14.29%
Elsevier	Elsevier, 1, 14.29% Elsevier 1 publication, 14.29%
	1 2

We do not take into account publications without a DOI.
Statistics recalculated weekly.

Are you a researcher?

Create a profile to get free access to personal recommendations for colleagues and new articles.

Metrics

Cite this

GOST |

Cite this

GOST Copy

Machikhin A. et al. Spatio-temporal segmentation of image sequences for non-invasive analysis of cardiovascular structure and function in Whitefish embryos // Micron. 2022. Vol. 163. p. 103360.

GOST all authors (up to 50) Copy

Machikhin A., Guryleva A., Selyukov A. G., Burlakov A. B., Bukova V., Khokhlov D. D., EFREMOVA E. V., Rudenko E. E. Spatio-temporal segmentation of image sequences for non-invasive analysis of cardiovascular structure and function in Whitefish embryos // Micron. 2022. Vol. 163. p. 103360.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1016/j.micron.2022.103360

UR - https://doi.org/10.1016/j.micron.2022.103360

TI - Spatio-temporal segmentation of image sequences for non-invasive analysis of cardiovascular structure and function in Whitefish embryos

T2 - Micron

AU - Machikhin, Alexander

AU - Guryleva, A.V.

AU - Selyukov, A G

AU - Burlakov, A B

AU - Bukova, V.I.

AU - Khokhlov, Demid D

AU - EFREMOVA, E. V.

AU - Rudenko, E. E.

PY - 2022

DA - 2022/12/01

PB - Elsevier

SP - 103360

VL - 163

PMID - 36274447

SN - 0968-4328

SN - 1878-4291

ER -

BibTex

Cite this

BibTex (up to 50 authors) Copy

@article{2022_Machikhin,

author = {Alexander Machikhin and A.V. Guryleva and A G Selyukov and A B Burlakov and V.I. Bukova and Demid D Khokhlov and E. V. EFREMOVA and E. E. Rudenko},

title = {Spatio-temporal segmentation of image sequences for non-invasive analysis of cardiovascular structure and function in Whitefish embryos},

journal = {Micron},

year = {2022},

volume = {163},

publisher = {Elsevier},

month = {dec},

url = {https://doi.org/10.1016/j.micron.2022.103360},

pages = {103360},

doi = {10.1016/j.micron.2022.103360}

}

Publisher

Elsevier

Journal

Micron

scimago Q2

wos Q1

SJR

0.581

CiteScore

5.3

Impact factor

2.2

ISSN

09684328 (Print)

18784291 (Electronic)

Profiles