Optimized semi-quantitative blot analysis in infection assays using the Stain-Free technology
Publication type: Journal Article
Publication date: 2016-07-01
scimago Q3
wos Q3
SJR: 0.479
CiteScore: 3.9
Impact factor: 1.9
ISSN: 01677012, 18728359
PubMed ID:
27150675
Molecular Biology
Microbiology (medical)
Microbiology
Abstract
Western blots are a commonly used method for protein detection and quantification in biological samples. Compensation of loading variations is achieved by housekeeping protein (HKP) normalization and/or total protein normalization (TPN). However, under infection conditions, HKP normalization, traditionally used in cell biology for quantification of western blots, can be problematic. Binding of microbes to target cells via specific receptors can induce signal transduction events resulting in drastic changes in the level of expression of HKPs. Additionally, samples collected after infection assays will include cellular and microbial proteins altering the analysis with TPN. Here we demonstrate under experimental infection conditions, how a reliable semi-quantitative analysis of proteins in western blots can be achieved using the Stain-Free technology.
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17
Total citations:
17
Citations from 2024:
2
(11.76%)
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BibTex
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GOST
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Zeitler A. F. et al. Optimized semi-quantitative blot analysis in infection assays using the Stain-Free technology // Journal of Microbiological Methods. 2016. Vol. 126. pp. 38-41.
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Zeitler A. F., Gerrer K. H., HAAS R. J., Jiménez Soto L. F. Optimized semi-quantitative blot analysis in infection assays using the Stain-Free technology // Journal of Microbiological Methods. 2016. Vol. 126. pp. 38-41.
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RIS
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TY - JOUR
DO - 10.1016/j.mimet.2016.04.016
UR - https://doi.org/10.1016/j.mimet.2016.04.016
TI - Optimized semi-quantitative blot analysis in infection assays using the Stain-Free technology
T2 - Journal of Microbiological Methods
AU - Zeitler, Anna F
AU - Gerrer, Katrin H
AU - HAAS, R. J.
AU - Jiménez Soto, Luisa Fernanda
PY - 2016
DA - 2016/07/01
PB - Elsevier
SP - 38-41
VL - 126
PMID - 27150675
SN - 0167-7012
SN - 1872-8359
ER -
Cite this
BibTex (up to 50 authors)
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@article{2016_Zeitler,
author = {Anna F Zeitler and Katrin H Gerrer and R. J. HAAS and Luisa Fernanda Jiménez Soto},
title = {Optimized semi-quantitative blot analysis in infection assays using the Stain-Free technology},
journal = {Journal of Microbiological Methods},
year = {2016},
volume = {126},
publisher = {Elsevier},
month = {jul},
url = {https://doi.org/10.1016/j.mimet.2016.04.016},
pages = {38--41},
doi = {10.1016/j.mimet.2016.04.016}
}