Synthesis, characterization, and biological studies of novel organometallic compounds of Re(I) with substituted (E)-2-(2-benzylidenehydrazinyl)-3-nitropyridine

Organometallic Re(I) complexes and substituted (E)-2-(2-benzylidenehydrazinyl)-3-nitropyridine were effectively synthesized and characterized using a variety of spectroscopic techniques. Their interaction with CT-DNA was investigated through viscosity measurements and absorption titration tests. An intercalation binding mode was discovered, and molecular docking analysis confirmed this finding. Additionally, protein-binding affinity was assessed by BSA binding assays. Tests of the synthesized compounds' antibacterial activity against three Gram-negative and two Gram-positive strains showed that Re(I) complexes exhibit more potent antibacterial activities than free ligands. SwissADME was used to predict pharmacokinetic parameters to assess drug-likeness. DFT computations were used to calculate the compounds' molecular orbital energies. Furthermore, the anticancer potential was tested against MCF-7 cells, and cytotoxicity was evaluated using a brine shrimp lethality assay. The IC50 values were utilized to assess the compounds' anticancer activity and compared to standard drugs.