A new fluorescence turn-on probe for biothiols based on photoinduced electron transfer and its application in living cells
Publication type: Journal Article
Publication date: 2016-09-01
scimago Q2
wos Q1
SJR: 0.664
CiteScore: 8.5
Impact factor: 4.6
ISSN: 13861425, 18733557
PubMed ID:
27203232
Spectroscopy
Analytical Chemistry
Atomic and Molecular Physics, and Optics
Instrumentation
Abstract
A new biothiol-selective fluorescent probe 1 based on photoinduced electron transfer (PET) mechanism was designed and synthesized. The UV-Vis absorption and fluorescent emission properties of probe 1 towards various analytes were studied in detail. The probe exhibited a large stokes shift (~200nm) after reacted with biothiols and could selectively detect cysteine (Cys) in dimethyl sulfoxide (DMSO)/H2O solution (9:1, v/v, 10mM phosphate buffer saline, pH3.5) over glutathione (GSH), homocysteine (Hcy) and other analytes with a detection limit of 0.117μM. In addition, probe 1 responded well to GSH, Hcy and Cys in the same above solution with pH5.5 and got the detection limits of 0.151μM, 0.128μM and 0.037μM, respectively. Probe 1 was of very low cytotoxicity and successfully applied for imaging of thiols in living cells.
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14
Total citations:
14
Citations from 2024:
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(7%)
The most citing journal
Citations in journal:
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GOST
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Wang J. et al. A new fluorescence turn-on probe for biothiols based on photoinduced electron transfer and its application in living cells // Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2016. Vol. 166. pp. 31-37.
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Wang J., Zhou C., Zhang J., Liu X., Wang Q., Zhu X. A new fluorescence turn-on probe for biothiols based on photoinduced electron transfer and its application in living cells // Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2016. Vol. 166. pp. 31-37.
Cite this
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TY - JOUR
DO - 10.1016/j.saa.2016.05.004
UR - https://doi.org/10.1016/j.saa.2016.05.004
TI - A new fluorescence turn-on probe for biothiols based on photoinduced electron transfer and its application in living cells
T2 - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
AU - Wang, Jianxi
AU - Zhou, Cheng
AU - Zhang, Jianjian
AU - Liu, Xiaoyan
AU - Wang, Qin
AU - Zhu, Xinyue
PY - 2016
DA - 2016/09/01
PB - Elsevier
SP - 31-37
VL - 166
PMID - 27203232
SN - 1386-1425
SN - 1873-3557
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2016_Wang,
author = {Jianxi Wang and Cheng Zhou and Jianjian Zhang and Xiaoyan Liu and Qin Wang and Xinyue Zhu},
title = {A new fluorescence turn-on probe for biothiols based on photoinduced electron transfer and its application in living cells},
journal = {Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy},
year = {2016},
volume = {166},
publisher = {Elsevier},
month = {sep},
url = {https://doi.org/10.1016/j.saa.2016.05.004},
pages = {31--37},
doi = {10.1016/j.saa.2016.05.004}
}