Green synthesis of red-emitting carbon dots for bioimaging, sensing, and antibacterial applications

It is a highly desirable and formidable challenge to synthesize carbon dots with long-wavelength emission using green synthesis. In this work, we explored red-emitting carbon dots (rCDs) via a hydrothermal strategy and their multifunctional application for bioimaging in vivo/vitro, curcumin sensing, and antibacterial materials. As-prepared rCDs were water-soluble and monodispersed with an average diameter of 2.34 nm. Significantly, these rCDs exhibited low toxicity and outstanding biocompatibility, which was consistent with the excellent bioimaging performance in living cells, zebrafish, and nude mice, providing them a promising prospect for clinical applications. Meanwhile, the obtained rCDs were also used as a fluorescent probe for sensitive detection of curcumin in a wide linear range of 0.03–135.73 μM with a limit of detection of 29.37 nM. Furthermore, quaternized rCDs were designed and used as antibacterial material with minimum inhibitory concentrations against Staphylococcus aureus and Escherichia coli of 0.15 mg/mL and 0.5 mg/mL, respectively, which advanced the development of novel antibacterial agents and broadened the applications of red-emitting CDs. Therefore, this work provided multifunctional CDs with red emission for use in the fields of biological imaging, fluorescence sensing, and antibacterial materials.