Talanta, volume 278, pages 126441
Rapid, portable, and sensitive detection of CaMV35S by RPA-CRISPR/Cas12a-G4 colorimetric assays with high accuracy deep learning object recognition and classification
Xuheng Li
1
,
Meilin Liu
1
,
Dianhui Men
1
,
Yi Duan
1
,
Liyuan Deng
1
,
Shiying Zhou
1
,
Jingzhou Hou
1, 2
,
Changjun Hou
1, 3
2
Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing, 401331, PR China
|
Publication type: Journal Article
Publication date: 2024-10-01
Abstract
Fast, sensitive, and portable detection of genetic modification contributes to agricultural security and food safety. Here, we developed RPA-CRISPR/Cas12a-G-quadruplex colorimetric assays that can combine with intelligent recognition by deep learning algorithms to achieve sensitive, rapid, and portable detection of the CaMV35S promoter. When the crRNA-Cas12a complex recognizes the RPA amplification product, Cas12 cleaves the G-quadruplex, causing the G4-Hemin complex to lose its peroxide mimetic enzyme function and be unable to catalyze the conversion of ABTS
Found
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