Talanta, volume 281, pages 126903
Enhanced performance of impedimetric immunosensors to detect SARS-CoV-2 with bare gold nanoparticles and graphene acetic acid
Rafael C Hensel
1
,
Biagio Di Vizio
2
,
Elsa María Materón
3
,
Flavio M. Shimizu
4
,
Monara Kaélle Sérvulo Cruz Angelim
5
,
Gabriela Fabiano De Souza
6
,
José Luiz Proença-Módena
6
,
Pedro M. Moraes-Vieira
5
,
R.B. Azevedo
7
,
Lucio Litti
2
,
S. Agnoli
2
,
S Casalini
2
,
7
Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil.
Publication type: Journal Article
Publication date: 2025-01-01
Abstract
Immunosensors based on electrical impedance spectroscopy allow for label-free, real-time detection of biologically relevant molecules and pathogens, without requiring electro-active materials. Here, we investigate the influence of bare gold nanoparticles (AuNPs), synthesized via laser ablation in solution, on the performance of an impedimetric immunosensor for detecting severe acute respiratory syndrome coronavirus (SARS-CoV-2). Graphene acetic acid (GAA) was used in the active layer for immobilizing anti-SARS-CoV-2 antibodies, owing to its high density of carboxylic groups. Immunosensors incorporating AuNPs exhibited superior performance compared to those relying solely on GAA, achieving a limit of detection (LoD) of 3 x 10
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