Open Access
Acute gene inactivation in the adult mouse liver using the CRISPR-Cas9 technology
Publication type: Journal Article
Publication date: 2021-09-01
scimago Q1
wos Q4
SJR: 0.925
CiteScore: 2.5
Impact factor: 1.3
ISSN: 26661667
PubMed ID:
34189476
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Neuroscience
Abstract
Genetic manipulation in mice allows the discovery of gene function and biological mechanisms in vivo. The widely used Cre/LoxP system usually takes months to years especially when starting with the production of floxed alleles of a new gene of interest (GOI). Here, we describe a protocol using the CRISPR-Cas9 system to acutely inactivate the GOI in adult mice. This protocol enables hepatocyte-specific gene editing within 4 weeks in adult mice and avoids compensatory effects of traditional gene inactivation initiated during various developmental stages. For complete details on the use and execution of this protocol, please refer to Wang et al. (2020).
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Citations from 2024:
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GOST
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Wang X., Xu B., Chen X. Acute gene inactivation in the adult mouse liver using the CRISPR-Cas9 technology // STAR Protocols. 2021. Vol. 2. No. 3. p. 100611.
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Wang X., Xu B., Chen X. Acute gene inactivation in the adult mouse liver using the CRISPR-Cas9 technology // STAR Protocols. 2021. Vol. 2. No. 3. p. 100611.
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RIS
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TY - JOUR
DO - 10.1016/j.xpro.2021.100611
UR - https://doi.org/10.1016/j.xpro.2021.100611
TI - Acute gene inactivation in the adult mouse liver using the CRISPR-Cas9 technology
T2 - STAR Protocols
AU - Wang, Xiao
AU - Xu, Bo-lin
AU - Chen, Xiaowei
PY - 2021
DA - 2021/09/01
PB - Elsevier
SP - 100611
IS - 3
VL - 2
PMID - 34189476
SN - 2666-1667
ER -
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BibTex (up to 50 authors)
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@article{2021_Wang,
author = {Xiao Wang and Bo-lin Xu and Xiaowei Chen},
title = {Acute gene inactivation in the adult mouse liver using the CRISPR-Cas9 technology},
journal = {STAR Protocols},
year = {2021},
volume = {2},
publisher = {Elsevier},
month = {sep},
url = {https://doi.org/10.1016/j.xpro.2021.100611},
number = {3},
pages = {100611},
doi = {10.1016/j.xpro.2021.100611}
}
Cite this
MLA
Copy
Wang, Xiao, et al. “Acute gene inactivation in the adult mouse liver using the CRISPR-Cas9 technology.” STAR Protocols, vol. 2, no. 3, Sep. 2021, p. 100611. https://doi.org/10.1016/j.xpro.2021.100611.