The labelling of an axonal membrane component with 4-(N-maleimido) benzyltrimethylammonium, a reagent capable of affinity-labelling the α-subunit of the nicotinic acetylcholine receptor
Publication type: Journal Article
Publication date: 1983-02-01
scimago Q2
wos Q3
SJR: 0.748
CiteScore: 4.9
Impact factor: 2.2
ISSN: 0006291X, 10902104
PubMed ID:
6830602
Biochemistry
Molecular Biology
Cell Biology
Biophysics
Abstract
Membrane vesicles, isolated from crustacean axons, were treated, following disulfide reduction, with 3H-NEM or with 3H-MBTA. SDS polyacrylamide gel electrophoresis showed that exposure to NEM (a nonspecific thiol reagent) resulted in the labelling of several peptide bands, while with MBTA only a single band with a molecular weight of 50,000 was labelled. Reaction with MBTA (believed to be a specific label for the nicotinic acetylcholine receptor) could be largely prevented by preincubation with d-tubocurarine or bromacetylcholine.
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Mautner H. G., Cole J. T., Belew M. A. The labelling of an axonal membrane component with 4-(N-maleimido) benzyltrimethylammonium, a reagent capable of affinity-labelling the α-subunit of the nicotinic acetylcholine receptor // Biochemical and Biophysical Research Communications. 1983. Vol. 111. No. 1. pp. 61-66.
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Mautner H. G., Cole J. T., Belew M. A. The labelling of an axonal membrane component with 4-(N-maleimido) benzyltrimethylammonium, a reagent capable of affinity-labelling the α-subunit of the nicotinic acetylcholine receptor // Biochemical and Biophysical Research Communications. 1983. Vol. 111. No. 1. pp. 61-66.
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TY - JOUR
DO - 10.1016/s0006-291x(83)80117-x
UR - https://doi.org/10.1016/s0006-291x(83)80117-x
TI - The labelling of an axonal membrane component with 4-(N-maleimido) benzyltrimethylammonium, a reagent capable of affinity-labelling the α-subunit of the nicotinic acetylcholine receptor
T2 - Biochemical and Biophysical Research Communications
AU - Mautner, Henry G
AU - Cole, James T.
AU - Belew, Margaret A
PY - 1983
DA - 1983/02/01
PB - Elsevier
SP - 61-66
IS - 1
VL - 111
PMID - 6830602
SN - 0006-291X
SN - 1090-2104
ER -
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@article{1983_Mautner,
author = {Henry G Mautner and James T. Cole and Margaret A Belew},
title = {The labelling of an axonal membrane component with 4-(N-maleimido) benzyltrimethylammonium, a reagent capable of affinity-labelling the α-subunit of the nicotinic acetylcholine receptor},
journal = {Biochemical and Biophysical Research Communications},
year = {1983},
volume = {111},
publisher = {Elsevier},
month = {feb},
url = {https://doi.org/10.1016/s0006-291x(83)80117-x},
number = {1},
pages = {61--66},
doi = {10.1016/s0006-291x(83)80117-x}
}
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MLA
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Mautner, Henry G., et al. “The labelling of an axonal membrane component with 4-(N-maleimido) benzyltrimethylammonium, a reagent capable of affinity-labelling the α-subunit of the nicotinic acetylcholine receptor.” Biochemical and Biophysical Research Communications, vol. 111, no. 1, Feb. 1983, pp. 61-66. https://doi.org/10.1016/s0006-291x(83)80117-x.