Open Access
A new and rapid colorimetric determination of acetylcholinesterase activity
Тип публикации: Journal Article
Дата публикации: 1961-07-01
SCImago Q1
Tоп 10% SCImago
WOS Q1
БС1
SJR: 1.687
CiteScore: 9.3
Impact factor: 6.5
ISSN: 00062952, 18732968
PubMed ID:
13726518
Краткое описание
Abstract A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described. The enzyme activity is measured by following the increase of yellow color produced from thiocholine when it reacts with dithiobisnitrobenzoate ion. It is based on coupling of these reactions: The latter reaction is rapid and the assay is sensitive (i.e. a 10 μ1 sample of blood is adequate). The use of a recorder has been most helpful, but is not essential. The method has been used to study the enzyme in human erythrocytes and homogenates of rat brain, kidney, lungs, liver and muscle tissue. Kinetic constants determined by this system for erythrocyte eholinesterase are presented. The data obtained with acetylthiocholine as substrate are similar to those with acetylcholine.
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Всего цитирований:
22577
Цитирований c 2025:
1911
(8.47%)
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ГОСТ
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Ellman G. L. et al. A new and rapid colorimetric determination of acetylcholinesterase activity // Biochemical Pharmacology. 1961. Vol. 7. No. 2. pp. 88-95.
ГОСТ со всеми авторами (до 50)
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Ellman G. L., Courtney K. D., Andres V., Featherstone R. M. A new and rapid colorimetric determination of acetylcholinesterase activity // Biochemical Pharmacology. 1961. Vol. 7. No. 2. pp. 88-95.
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TY - JOUR
DO - 10.1016/0006-2952(61)90145-9
UR - https://doi.org/10.1016/0006-2952(61)90145-9
TI - A new and rapid colorimetric determination of acetylcholinesterase activity
T2 - Biochemical Pharmacology
AU - Ellman, G L
AU - Courtney, K D
AU - Andres, V
AU - Featherstone, R M
PY - 1961
DA - 1961/07/01
PB - Elsevier
SP - 88-95
IS - 2
VL - 7
PMID - 13726518
SN - 0006-2952
SN - 1873-2968
ER -
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BibTex (до 50 авторов)
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@article{1961_Ellman,
author = {G L Ellman and K D Courtney and V Andres and R M Featherstone},
title = {A new and rapid colorimetric determination of acetylcholinesterase activity},
journal = {Biochemical Pharmacology},
year = {1961},
volume = {7},
publisher = {Elsevier},
month = {jul},
url = {https://doi.org/10.1016/0006-2952(61)90145-9},
number = {2},
pages = {88--95},
doi = {10.1016/0006-2952(61)90145-9}
}
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MLA
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Ellman, G. L., et al. “A new and rapid colorimetric determination of acetylcholinesterase activity.” Biochemical Pharmacology, vol. 7, no. 2, Jul. 1961, pp. 88-95. https://doi.org/10.1016/0006-2952(61)90145-9.
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