Trends in Biochemical Sciences, volume 14, issue 11, pages 450-454
Barnase and barstar: two small proteins to fold and fit together
1
R. W. Hartley is at the Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20897, USA.
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Publication type: Journal Article
Publication date: 1989-11-01
Journal:
Trends in Biochemical Sciences
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 13.8
ISSN: 03765067, 09680004, 13624326
Biochemistry
Molecular Biology
Abstract
Barnase and barstar are the extracellular ribonuclease and its intracellular inhibitor produced by Bacillus amyloliquefaciens. Both are small single-chain proteins and thus are suitable for application to the study of how a protein's sequence directs its fold. Barnase has neither disulfide bonds nor non-peptide components and unfolds reversibly in what closely approximates a two-state reaction. The genes for both these proteins have been cloned in E. coli. Expression of barstar is necessary to counter the lethal effect of expressed active barnase. Site-directed mutagenesis is being used to answer specific and general questions relating to protein folding and protein-protein interaction.
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Citations by publishers
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35
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5
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35
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Hartley R. W. Barnase and barstar: two small proteins to fold and fit together // Trends in Biochemical Sciences. 1989. Vol. 14. No. 11. pp. 450-454.
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Hartley R. W. Barnase and barstar: two small proteins to fold and fit together // Trends in Biochemical Sciences. 1989. Vol. 14. No. 11. pp. 450-454.
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TY - JOUR
DO - 10.1016/0968-0004(89)90104-7
UR - https://doi.org/10.1016/0968-0004(89)90104-7
TI - Barnase and barstar: two small proteins to fold and fit together
T2 - Trends in Biochemical Sciences
AU - Hartley, Robert W.
PY - 1989
DA - 1989/11/01 00:00:00
PB - Elsevier
SP - 450-454
IS - 11
VL - 14
SN - 0376-5067
SN - 0968-0004
SN - 1362-4326
ER -
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@article{1989_Hartley,
author = {Robert W. Hartley},
title = {Barnase and barstar: two small proteins to fold and fit together},
journal = {Trends in Biochemical Sciences},
year = {1989},
volume = {14},
publisher = {Elsevier},
month = {nov},
url = {https://doi.org/10.1016/0968-0004(89)90104-7},
number = {11},
pages = {450--454},
doi = {10.1016/0968-0004(89)90104-7}
}
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MLA
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Hartley, Robert W.. “Barnase and barstar: two small proteins to fold and fit together.” Trends in Biochemical Sciences, vol. 14, no. 11, Nov. 1989, pp. 450-454. https://doi.org/10.1016/0968-0004(89)90104-7.