Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and frit-FAB LC/MS
1
Central Research Laboratories, Ajinomoto Co., Inc., Kanagawa, Japan.
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2
Central Research Laboratories, Ajinomoto Co., Inc., 1–1 Suzuki-cho, Kawasaki-ku, Kawasaki, Kanagawa 210, Japan
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Publication type: Journal Article
Publication date: 1990-12-01
scimago Q2
wos Q3
SJR: 0.748
CiteScore: 4.9
Impact factor: 2.2
ISSN: 0006291X, 10902104
PubMed ID:
2260975
Biochemistry
Molecular Biology
Cell Biology
Biophysics
Abstract
Incorrectness of the amino acid sequence of bovine serum albumin (BSA) was suggested from the observed molecular weight of BSA obtained by electrospray ionization mass spectrometry (ESIMS). Lack of a tyrosine residue in the position of 156th was found rapidly, by the combination of frit-fast atom bombardment mass spectrometry/liquid chromatography (Frit-FAB LC/MS), automated Edman degradation and tandem mass spectrometry (MS). Then it turned out that BSA is composed of 583 amino acid residues, and that its average molecular weight is not 66267.1, and it is corrected to 66430.3. Moreover the amino acid sequence of the positions of 94th and 95th was corrected to -QE- by using automated Edman degradation method.
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486
Total citations:
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Citations from 2025:
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(3.71%)
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GOST
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Hirayama K. et al. Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and frit-FAB LC/MS // Biochemical and Biophysical Research Communications. 1990. Vol. 173. No. 2. pp. 639-646.
GOST all authors (up to 50)
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Hirayama K., Akashi S., Furuya M., FUKUHARA K. Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and frit-FAB LC/MS // Biochemical and Biophysical Research Communications. 1990. Vol. 173. No. 2. pp. 639-646.
Cite this
RIS
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TY - JOUR
DO - 10.1016/S0006-291X(05)80083-X
UR - https://doi.org/10.1016/S0006-291X(05)80083-X
TI - Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and frit-FAB LC/MS
T2 - Biochemical and Biophysical Research Communications
AU - Hirayama, Kazuo
AU - Akashi, Satoko
AU - Furuya, Mami
AU - FUKUHARA, Ken-ichi
PY - 1990
DA - 1990/12/01
PB - Elsevier
SP - 639-646
IS - 2
VL - 173
PMID - 2260975
SN - 0006-291X
SN - 1090-2104
ER -
Cite this
BibTex (up to 50 authors)
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@article{1990_Hirayama,
author = {Kazuo Hirayama and Satoko Akashi and Mami Furuya and Ken-ichi FUKUHARA},
title = {Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and frit-FAB LC/MS},
journal = {Biochemical and Biophysical Research Communications},
year = {1990},
volume = {173},
publisher = {Elsevier},
month = {dec},
url = {https://doi.org/10.1016/S0006-291X(05)80083-X},
number = {2},
pages = {639--646},
doi = {10.1016/S0006-291X(05)80083-X}
}
Cite this
MLA
Copy
Hirayama, Kazuo, et al. “Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and frit-FAB LC/MS.” Biochemical and Biophysical Research Communications, vol. 173, no. 2, Dec. 1990, pp. 639-646. https://doi.org/10.1016/S0006-291X(05)80083-X.