Chapter 37 Spectra of Fluorescent Dyes Used in Flow Cytometry
1
Molecular Probes, Inc. Eugene, Oregon 97402
|
Publication type: Book Chapter
Publication date: 1994-01-01
scimago Q4
SJR: 0.296
CiteScore: 2.2
Impact factor: —
ISSN: 0091679X
PubMed ID:
7533258
Abstract
This chapter reviews the factors that go into the selection of dyes for use in flow cytometry, identifies the principal dyes being used, and compares their spectral properties. It also reviews some of the recent developments in the field related to dye development and applications of new probes in flow cytometry. Flow cytometry uses a relatively small set of dyes for immunochemistry and nucleic acid detection, most of which have been known and used reliably in flow cytometry for several years. These can usually be combined to make simultaneous two-color measurements of multiple cell-surface antigens and nucleic acid content. Because of the overlap of dye spectra and difficulties in finding dyes with substantial Stokes shifts that can be excited by the argon laser, simultaneous three-color or more detection can be more difficult. A basic knowledge of the factors that go into producing the fluorescent signal, including the spectra of dyes and their overlap, is necessary in planning multicolor experiments.
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GOST
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Haugland R. P. Chapter 37 Spectra of Fluorescent Dyes Used in Flow Cytometry // Methods in Cell Biology. 1994. Vol. 42 Pt B. pp. 641-663.
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Haugland R. P. Chapter 37 Spectra of Fluorescent Dyes Used in Flow Cytometry // Methods in Cell Biology. 1994. Vol. 42 Pt B. pp. 641-663.
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TY - GENERIC
DO - 10.1016/S0091-679X(08)61100-0
UR - https://doi.org/10.1016/S0091-679X(08)61100-0
TI - Chapter 37 Spectra of Fluorescent Dyes Used in Flow Cytometry
T2 - Methods in Cell Biology
AU - Haugland, Richard P.
PY - 1994
DA - 1994/01/01
PB - Elsevier
SP - 641-663
VL - 42 Pt B
PMID - 7533258
SN - 0091-679X
ER -
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@incollection{1994_Haugland,
author = {Richard P. Haugland},
title = {Chapter 37 Spectra of Fluorescent Dyes Used in Flow Cytometry},
publisher = {Elsevier},
year = {1994},
volume = {42 Pt B},
pages = {641--663},
month = {jan}
}