Enzymatic synthesis of no-carrier-added 6-[18F]fluoro-l-dopa with β-tyrosinase
3
Department of Biofunctional Research, National Institute for Longevity Sciences, Gengo, Morioka-cho, Obu, Aichi 474-8522, Japan
|
4
Research Laboratory, Ikeda Food Research Co., Ltd., 95-7 Minooki-cho, Fukuyama, Hiroshima 721-8558, Japan
|
Publication type: Journal Article
Publication date: 1999-06-01
scimago Q2
wos Q2
SJR: 0.408
CiteScore: 3.2
Impact factor: 1.8
ISSN: 09698043, 18729800
PubMed ID:
10391715
Radiation
Abstract
An enzymatic synthesis of nca 6-[18F]fluoro-L-dopa has been developed. The process consists of a chemical synthesis of 4-[18F]fluorocatechol and its enzymatic reaction with beta-tyrosinase. The 4-[18F]fluorocatechol was prepared by nucleophilic aromatic substitution of the NO2 group on 6-nitroveratoraldehyde with [K/222]+18F-, followed by decarbonylation with tris(triphenylphosphine) rhodium(I) chloride and hydrolysis with hydroiodic acid. By C18 Sep-Pak purification, 4-[18F]fluorocatechol was obtained in ethanol with a radiochemical yield of 9.2%. An enzymatic reaction of 4-[18F]fluorocatechol, ammonium and pyruvate catalyzed by beta-tyrosinase in an ethanolic Tris-HCl buffer (pH 9.0) containing ascorbate gave within 5 min 6-[18F]fluoro-L-dopa with an approximate radiochemical yield of 60% without any isomers. The deproteinized reaction mixture was applied to a preparative reverse phase column, and the radiochemically and enantiomerically pure 6-[18F]fluoro-L-dopa was obtained with a radiochemical yield of 2.0% based on [18F]F- (decay-corrected). The synthesis time was 150 min from the EOB and the specific activity was > 200 GBq/micromol.
Found
Nothing found, try to update filter.
Found
Nothing found, try to update filter.
Top-30
Journals
|
1
2
3
4
|
|
|
Applied Radiation and Isotopes
4 publications, 20%
|
|
|
Journal of Labelled Compounds and Radiopharmaceuticals
3 publications, 15%
|
|
|
Radiochimica Acta
2 publications, 10%
|
|
|
Russian Chemical Reviews
1 publication, 5%
|
|
|
Journal of Bioscience and Bioengineering
1 publication, 5%
|
|
|
EJNMMI Radiopharmacy and Chemistry
1 publication, 5%
|
|
|
Medicinal Chemistry Research
1 publication, 5%
|
|
|
Russian Chemical Bulletin
1 publication, 5%
|
|
|
Chemical Reviews
1 publication, 5%
|
|
|
BioMed Research International
1 publication, 5%
|
|
|
Mendeleev Communications
1 publication, 5%
|
|
|
1
2
3
4
|
Publishers
|
1
2
3
4
5
6
|
|
|
Elsevier
6 publications, 30%
|
|
|
Springer Nature
3 publications, 15%
|
|
|
Wiley
3 publications, 15%
|
|
|
Walter de Gruyter
2 publications, 10%
|
|
|
Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 5%
|
|
|
American Chemical Society (ACS)
1 publication, 5%
|
|
|
Hindawi Limited
1 publication, 5%
|
|
|
1
2
3
4
5
6
|
- We do not take into account publications without a DOI.
- Statistics recalculated weekly.
Are you a researcher?
Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
20
Total citations:
20
Citations from 2025:
0
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Kaneko S. et al. Enzymatic synthesis of no-carrier-added 6-[18F]fluoro-l-dopa with β-tyrosinase // Applied Radiation and Isotopes. 1999. Vol. 50. No. 6. pp. 1025-1032.
GOST all authors (up to 50)
Copy
Kaneko S., ISHIWATA K., Hatano K., Omura H., Ito K., Senda M. Enzymatic synthesis of no-carrier-added 6-[18F]fluoro-l-dopa with β-tyrosinase // Applied Radiation and Isotopes. 1999. Vol. 50. No. 6. pp. 1025-1032.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1016/S0969-8043(98)00173-0
UR - https://doi.org/10.1016/S0969-8043(98)00173-0
TI - Enzymatic synthesis of no-carrier-added 6-[18F]fluoro-l-dopa with β-tyrosinase
T2 - Applied Radiation and Isotopes
AU - Kaneko, Shoji
AU - ISHIWATA, KIICHI
AU - Hatano, Kentaro
AU - Omura, Hironori
AU - Ito, Kengo
AU - Senda, M.
PY - 1999
DA - 1999/06/01
PB - Elsevier
SP - 1025-1032
IS - 6
VL - 50
PMID - 10391715
SN - 0969-8043
SN - 1872-9800
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{1999_Kaneko,
author = {Shoji Kaneko and KIICHI ISHIWATA and Kentaro Hatano and Hironori Omura and Kengo Ito and M. Senda},
title = {Enzymatic synthesis of no-carrier-added 6-[18F]fluoro-l-dopa with β-tyrosinase},
journal = {Applied Radiation and Isotopes},
year = {1999},
volume = {50},
publisher = {Elsevier},
month = {jun},
url = {https://doi.org/10.1016/S0969-8043(98)00173-0},
number = {6},
pages = {1025--1032},
doi = {10.1016/S0969-8043(98)00173-0}
}
Cite this
MLA
Copy
Kaneko, Shoji, et al. “Enzymatic synthesis of no-carrier-added 6-[18F]fluoro-l-dopa with β-tyrosinase.” Applied Radiation and Isotopes, vol. 50, no. 6, Jun. 1999, pp. 1025-1032. https://doi.org/10.1016/S0969-8043(98)00173-0.