Archives of Biochemistry and Biophysics, volume 728, pages 109375
Anticancer therapeutic potential of phosphorylated galactosylated chitosan against N-nitrosodiethyl amine-induced hepatocarcinogenesis
U Anushree
1
,
Sachin Shetty
1
,
S. D. Kulkarni
2
,
Harish Kumar B
3
,
K Sreedhara R. Pai
3
,
Jesil M Aranjani
4
,
Rajesh Kumar
5
,
Sanjay Bharati
1
Publication type: Journal Article
Publication date: 2022-10-01
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 3.9
ISSN: 00039861, 10960384
Biochemistry
Molecular Biology
Biophysics
Abstract
Chitosan is a natural polyfunctional polymer that can be modified to achieve compounds with tailored properties for targeting and treating different cancers. In this study, we report the development and anticancer potential of phosphorylated galactosylated chitosan (PGC). The synthesized compound was characterized by FT-IR, NMR, and mass spectroscopy. The interaction of PGC with asialoglycoprotein receptors (ASGPR) and cellular internalization in HepG2 cells was studied using in silico and uptake studies respectively. PGC was evaluated for its metal chelating, ferric ion reducing, superoxide, and lipid peroxide (LPO) inhibiting potential. Further, anticancer therapeutic potential of PGC was evaluated against N-nitrosodiethylamine (NDEA)-induced hepatocellular carcinoma in a mice model. After development of cancer, PGC was administered to the treatment group (0.5 mg/kg bw, intravenously), once a week for 4 weeks. Characterization studies of PGC revealed successful phosphorylation and galactosylation of chitosan. A strong interaction of PGC with ASGP-receptors was predicted by computational studies and cellular internalization studies demonstrated 98.76 ± 0.53% uptake of PGC in the HepG2 cells. A good metal chelating, ferric ion reducing, and free radical scavenging activity was demonstrated by PGC. The anticancer therapeutic potential of PGC was evident from the observation that PGC treatment increased number of tumor free animals (50%) (6/12) and significantly (p ≤ 0.05) lowered tumor multiplicity as compared to untreated tumor group.
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Anushree U. et al. Anticancer therapeutic potential of phosphorylated galactosylated chitosan against N-nitrosodiethyl amine-induced hepatocarcinogenesis // Archives of Biochemistry and Biophysics. 2022. Vol. 728. p. 109375.
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Anushree U., Shetty S., Kulkarni S. D., B H. K., Pai K. S. R., Aranjani J. M., Kumar R., Bharati S. Anticancer therapeutic potential of phosphorylated galactosylated chitosan against N-nitrosodiethyl amine-induced hepatocarcinogenesis // Archives of Biochemistry and Biophysics. 2022. Vol. 728. p. 109375.
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TY - JOUR
DO - 10.1016/j.abb.2022.109375
UR - https://doi.org/10.1016/j.abb.2022.109375
TI - Anticancer therapeutic potential of phosphorylated galactosylated chitosan against N-nitrosodiethyl amine-induced hepatocarcinogenesis
T2 - Archives of Biochemistry and Biophysics
AU - Anushree, U
AU - Shetty, Sachin
AU - Kulkarni, S. D.
AU - B, Harish Kumar
AU - Pai, K Sreedhara R.
AU - Aranjani, Jesil M
AU - Kumar, Rajesh
AU - Bharati, Sanjay
PY - 2022
DA - 2022/10/01 00:00:00
PB - Elsevier
SP - 109375
VL - 728
SN - 0003-9861
SN - 1096-0384
ER -
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@article{2022_Anushree,
author = {U Anushree and Sachin Shetty and S. D. Kulkarni and Harish Kumar B and K Sreedhara R. Pai and Jesil M Aranjani and Rajesh Kumar and Sanjay Bharati},
title = {Anticancer therapeutic potential of phosphorylated galactosylated chitosan against N-nitrosodiethyl amine-induced hepatocarcinogenesis},
journal = {Archives of Biochemistry and Biophysics},
year = {2022},
volume = {728},
publisher = {Elsevier},
month = {oct},
url = {https://doi.org/10.1016/j.abb.2022.109375},
pages = {109375},
doi = {10.1016/j.abb.2022.109375}
}