volume 517 issue 3 pages 463-469

Projection structures reveal the position of the DNA within DNA-Dps Co-crystals

Publication typeJournal Article
Publication date2019-09-01
scimago Q2
wos Q3
SJR0.748
CiteScore4.9
Impact factor2.2
ISSN0006291X, 10902104
Biochemistry
Molecular Biology
Cell Biology
Biophysics
Abstract
One of the universal mechanisms for the response of Escherichia coli to stress is the increase of the synthesis of specific histone-like proteins that bind the DNA, Dps. As a result, two-and three-dimensional crystalline arrays may be observed in the cytoplasm of starving cells. Here, we determined the conditions to obtain very thin two-dimensional DNA-Dps co-crystals in vitro, and studied their projection structures, using electron microscopy. Analysis of the projection maps of the free Dps crystals revealed two lattice types: hexagonal and rectangular. We used the fluorescently labeled DNA to prove that the DNA is present within the co-crystals with Dps in vitro, and visualized its position using transmission electron microscopy. Molecular modeling confirmed the DNA position within the crystal. We have also suggested a structural model for the DNA-Dps co-crystal dissolving in the presence of Mg2+ ions.
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GOST |
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GOST Copy
Moiseenko A. V. et al. Projection structures reveal the position of the DNA within DNA-Dps Co-crystals // Biochemical and Biophysical Research Communications. 2019. Vol. 517. No. 3. pp. 463-469.
GOST all authors (up to 50) Copy
Moiseenko A. V., Loiko N., Tereshkina K., Danilova Y., Kovalenko V., Chertkov O., Feofanov A. V., Krupyanskii Y. F., Sokolova O. S. Projection structures reveal the position of the DNA within DNA-Dps Co-crystals // Biochemical and Biophysical Research Communications. 2019. Vol. 517. No. 3. pp. 463-469.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1016/j.bbrc.2019.07.103
UR - https://doi.org/10.1016/j.bbrc.2019.07.103
TI - Projection structures reveal the position of the DNA within DNA-Dps Co-crystals
T2 - Biochemical and Biophysical Research Communications
AU - Moiseenko, A V
AU - Loiko, Natalia
AU - Tereshkina, Ksenia
AU - Danilova, Yana
AU - Kovalenko, Vladislav
AU - Chertkov, Oleg
AU - Feofanov, A. V.
AU - Krupyanskii, Yurii F
AU - Sokolova, Olga S.
PY - 2019
DA - 2019/09/01
PB - Elsevier
SP - 463-469
IS - 3
VL - 517
PMID - 31376942
SN - 0006-291X
SN - 1090-2104
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2019_Moiseenko,
author = {A V Moiseenko and Natalia Loiko and Ksenia Tereshkina and Yana Danilova and Vladislav Kovalenko and Oleg Chertkov and A. V. Feofanov and Yurii F Krupyanskii and Olga S. Sokolova},
title = {Projection structures reveal the position of the DNA within DNA-Dps Co-crystals},
journal = {Biochemical and Biophysical Research Communications},
year = {2019},
volume = {517},
publisher = {Elsevier},
month = {sep},
url = {https://doi.org/10.1016/j.bbrc.2019.07.103},
number = {3},
pages = {463--469},
doi = {10.1016/j.bbrc.2019.07.103}
}
MLA
Cite this
MLA Copy
Moiseenko, A. V., et al. “Projection structures reveal the position of the DNA within DNA-Dps Co-crystals.” Biochemical and Biophysical Research Communications, vol. 517, no. 3, Sep. 2019, pp. 463-469. https://doi.org/10.1016/j.bbrc.2019.07.103.