Biosensors and Bioelectronics, volume 102, pages 80-86
Robust immunosensing system based on biotin-streptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6
Publication type: Journal Article
Publication date: 2018-04-01
Journal:
Biosensors and Bioelectronics
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 12.6
ISSN: 09565663, 18734235
General Medicine
Biophysics
Electrochemistry
Biotechnology
Biomedical Engineering
Abstract
Detection of a very low amount of cytokines such as interleukin-6 (IL-6) in clinical fluids is important in biomedical research and clinical applications. Here, we demonstrate spatially-localized ultrasensitive (femtogram mL-1) level detection of IL-6 in serum and in cell culture media. Our approach is based on a sandwich immunosensor fabricated on the surface of an optical fibre. Firstly, the biotinylated IL-6 capture antibody was immobilized on the fibre surface by biotin-streptavidin coupling. Then the fabricated fibre was used for capturing IL-6 followed by exposure to detection antibody which was labeled with the fluorescent magnetic nanoparticles to report the signal. A linear relationship between IL-6 concentration and the fluorescence signal was obtained in the range from 0.4pgmL-1 to 400pgmL-1 of IL-6, with the limit of detection down to 0.1pgmL-1. In addition, this optical fibre sensor was successfully applied for the localized detection of IL-6 with the spatial resolution of 200µm and a sample volume of 1µL. Finally, the performance of the fibre sensor was demonstrated by detection of IL-6 secreted by BV-2 cells with comparable performance of the conventional enzyme-linked immunosorbent assay (ELISA).
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Wiley
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IEEE
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Springer Nature
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Taylor & Francis
2 publications, 3.28%
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1 publication, 1.64%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 1.64%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Zhang K. et al. Robust immunosensing system based on biotin-streptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6 // Biosensors and Bioelectronics. 2018. Vol. 102. pp. 80-86.
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Zhang K., Liu G., Goldys E. M. Robust immunosensing system based on biotin-streptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6 // Biosensors and Bioelectronics. 2018. Vol. 102. pp. 80-86.
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TY - JOUR
DO - 10.1016/j.bios.2017.11.023
UR - https://doi.org/10.1016/j.bios.2017.11.023
TI - Robust immunosensing system based on biotin-streptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6
T2 - Biosensors and Bioelectronics
AU - Zhang, Kaixin
AU - Liu, Guozhen
AU - Goldys, Ewa M.
PY - 2018
DA - 2018/04/01 00:00:00
PB - Elsevier
SP - 80-86
VL - 102
SN - 0956-5663
SN - 1873-4235
ER -
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@article{2018_Zhang,
author = {Kaixin Zhang and Guozhen Liu and Ewa M. Goldys},
title = {Robust immunosensing system based on biotin-streptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6},
journal = {Biosensors and Bioelectronics},
year = {2018},
volume = {102},
publisher = {Elsevier},
month = {apr},
url = {https://doi.org/10.1016/j.bios.2017.11.023},
pages = {80--86},
doi = {10.1016/j.bios.2017.11.023}
}