Biosensors and Bioelectronics, volume 102, pages 80-86

Robust immunosensing system based on biotin-streptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6

Kaixin Zhang ¹

Guozhen Liu ^{2, 3}

Ewa M. Goldys ¹

Hide authors affiliations Show authors affiliations: 3 affiliations

ARC Centre of Excellence for Nanoscale Biophotonics (CNBP), Macquarie University, North Ryde 2109, Australia. |

ARC Centre of Excellence for Nanoscale Biophotonics (CNBP), Macquarie University, North Ryde 2109, Australia |

Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China |

Publication type: Journal Article

Publication date: 2018-04-01

Elsevier

Journal: Biosensors and Bioelectronics

Quartile SCImago

Quartile WOS

Impact factor: 12.6

ISSN: 09565663, 18734235

DOI: 10.1016/j.bios.2017.11.023

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General Medicine

Biophysics

Electrochemistry

Biotechnology

Biomedical Engineering

Abstract

Detection of a very low amount of cytokines such as interleukin-6 (IL-6) in clinical fluids is important in biomedical research and clinical applications. Here, we demonstrate spatially-localized ultrasensitive (femtogram mL-1) level detection of IL-6 in serum and in cell culture media. Our approach is based on a sandwich immunosensor fabricated on the surface of an optical fibre. Firstly, the biotinylated IL-6 capture antibody was immobilized on the fibre surface by biotin-streptavidin coupling. Then the fabricated fibre was used for capturing IL-6 followed by exposure to detection antibody which was labeled with the fluorescent magnetic nanoparticles to report the signal. A linear relationship between IL-6 concentration and the fluorescence signal was obtained in the range from 0.4pgmL-1 to 400pgmL-1 of IL-6, with the limit of detection down to 0.1pgmL-1. In addition, this optical fibre sensor was successfully applied for the localized detection of IL-6 with the spatial resolution of 200µm and a sample volume of 1µL. Finally, the performance of the fibre sensor was demonstrated by detection of IL-6 secreted by BV-2 cells with comparable performance of the conventional enzyme-linked immunosorbent assay (ELISA).

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Elsevier	Elsevier, 28, 45.9% Elsevier 28 publications, 45.9%
American Chemical Society (ACS)	American Chemical Society (ACS), 8, 13.11% American Chemical Society (ACS) 8 publications, 13.11%
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IEEE	IEEE, 3, 4.92% IEEE 3 publications, 4.92%
Springer Nature	Springer Nature, 2, 3.28% Springer Nature 2 publications, 3.28%
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Frontiers Media S.A.	Frontiers Media S.A., 1, 1.64% Frontiers Media S.A. 1 publication, 1.64%
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Zhang K. et al. Robust immunosensing system based on biotin-streptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6 // Biosensors and Bioelectronics. 2018. Vol. 102. pp. 80-86.

GOST all authors (up to 50) Copy

Zhang K., Liu G., Goldys E. M. Robust immunosensing system based on biotin-streptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6 // Biosensors and Bioelectronics. 2018. Vol. 102. pp. 80-86.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1016/j.bios.2017.11.023

UR - https://doi.org/10.1016/j.bios.2017.11.023

TI - Robust immunosensing system based on biotin-streptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6

T2 - Biosensors and Bioelectronics

AU - Zhang, Kaixin

AU - Liu, Guozhen

AU - Goldys, Ewa M.

PY - 2018

DA - 2018/04/01 00:00:00

PB - Elsevier

SP - 80-86

VL - 102

SN - 0956-5663

SN - 1873-4235

ER -

BibTex

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BibTex Copy

@article{2018_Zhang,

author = {Kaixin Zhang and Guozhen Liu and Ewa M. Goldys},

title = {Robust immunosensing system based on biotin-streptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6},

journal = {Biosensors and Bioelectronics},

year = {2018},

volume = {102},

publisher = {Elsevier},

month = {apr},

url = {https://doi.org/10.1016/j.bios.2017.11.023},

pages = {80--86},

doi = {10.1016/j.bios.2017.11.023}

}

Found error?

Publisher

Elsevier

Journal

Biosensors and Bioelectronics

Quartile SCImago

Quartile WOS

Impact factor

12.6

ISSN

09565663 (Print)
18734235 (Electronic)