Biosensors and Bioelectronics, volume 115, pages 7-13

Multiplexed aptasensor based on metal ions labels for simultaneous detection of multiple antibiotic residues in milk

Falan Li
Yemin Guo
Xiangyou Wang
Xia Sun
Publication typeJournal Article
Publication date2018-09-01
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor12.6
ISSN09565663, 18734235
General Medicine
Biophysics
Electrochemistry
Biotechnology
Biomedical Engineering
Abstract
A dual-target electrochemical aptasensor was developed for the simultaneous detection of multiple antibiotics based on metal ions as signal tracers and nanocomposites as signal amplification strategy. Metal ions such as Cd2+ and Pb2+ could generate distinct differential pulse voltammetry (DPV) peaks. When targets were present, kanamycin (KAN) and streptomycin (STR) as models, the KAN aptamer (KAP) and STR aptamer (STP) were released from their complementary strands, with more change of Cd2+ and Pb2+ corresponding to peak currents. At the same time, complementary strand of KAP (cKAP) and STP (cSTP) were linked with the poly (A) structure (cSTP-PolyA-cKAP) to increase their conformational freedom. Graphitized multi-walled carbon nanotubes (MWCNTGr) and carbon nanofibers-gold nanoparticles (CNFs-AuNPs) as a biosensor platform enhanced the surface area to capture a large amount of cSTP-PolyA-cKAP, thus amplifying the detection response. Under the optimal conditions, the aptasensor could detect KAN and STR as low as 74.50 pM and 36.45 pM respectively with the range from 0.1 to 100 nM and exhibited excellent selectively. Moreover, this aptasensor showed promising applications for the detection of other analytes by changing corresponding aptamers.

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GOST Copy
Li F. et al. Multiplexed aptasensor based on metal ions labels for simultaneous detection of multiple antibiotic residues in milk // Biosensors and Bioelectronics. 2018. Vol. 115. pp. 7-13.
GOST all authors (up to 50) Copy
Li F., Guo Y., Wang X., Sun X. Multiplexed aptasensor based on metal ions labels for simultaneous detection of multiple antibiotic residues in milk // Biosensors and Bioelectronics. 2018. Vol. 115. pp. 7-13.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1016/j.bios.2018.04.024
UR - https://doi.org/10.1016/j.bios.2018.04.024
TI - Multiplexed aptasensor based on metal ions labels for simultaneous detection of multiple antibiotic residues in milk
T2 - Biosensors and Bioelectronics
AU - Li, Falan
AU - Guo, Yemin
AU - Wang, Xiangyou
AU - Sun, Xia
PY - 2018
DA - 2018/09/01 00:00:00
PB - Elsevier
SP - 7-13
VL - 115
SN - 0956-5663
SN - 1873-4235
ER -
BibTex
Cite this
BibTex Copy
@article{2018_Li,
author = {Falan Li and Yemin Guo and Xiangyou Wang and Xia Sun},
title = {Multiplexed aptasensor based on metal ions labels for simultaneous detection of multiple antibiotic residues in milk},
journal = {Biosensors and Bioelectronics},
year = {2018},
volume = {115},
publisher = {Elsevier},
month = {sep},
url = {https://doi.org/10.1016/j.bios.2018.04.024},
pages = {7--13},
doi = {10.1016/j.bios.2018.04.024}
}
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