Biosensors and Bioelectronics, volume 167, pages 112433

Label-free immunodetection of α-synuclein by using a microfluidics coplanar electrolyte-gated organic field-effect transistor

Simona Ricci ¹

S Casalini ^{2, 3}

Vitaliy Parkula ⁴

Meenu Selvaraj ⁵

Gulseren Deniz Saygin ⁵

Pierpaolo Greco ⁵

F. Biscarini ^{4, 6}

Marta Mas-Torrent ¹

Hide authors affiliations Show authors affiliations: 6 affiliations

Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus de La Universitat Autònoma de Barcelona, Cerdanyola, 08193, Barcelona, Spain. |

Department of Chemical Sciences, University of Padua, via Francesco Marzolo 1, 35131, Padova, Italy |

⁴

University of Modena and Reggio Emilia, Via G. Campi 103, 41125, Modena, Italy |

⁵

Scriba Nanotecnologie srl, via di Corticella 183/8, 40128, Bologna, Italy |

⁶

Center for Translational Neurophysiology - Istituto Italiano di Tecnologia, Via Fossato di Mortara 17-19, 44100, Ferrara, Italy |

Publication type: Journal Article

Publication date: 2020-11-01

Elsevier

Journal: Biosensors and Bioelectronics

Quartile SCImago

Quartile WOS

Impact factor: 12.6

ISSN: 09565663, 18734235

DOI: 10.1016/j.bios.2020.112433

Copy DOI

General Medicine

Biophysics

Electrochemistry

Biotechnology

Biomedical Engineering

Abstract

The aggregation of α-synuclein is a critical event in the pathogenesis of neurological diseases, such as Parkinson or Alzheimer. Here, we present a label-free sensor based on an Electrolyte-Gated Organic Field-Effect Transistor (EGOFET) integrated with microfluidics that allows for the detection of amounts of α-synuclein in the range from 0.25 pM to 25 nM. The lower limit of detection (LOD) measures the potential of our integrated device as a tool for prognostics and diagnostics. In our device, the gate electrode is the effective sensing element as it is functionalised with anti-(α-synuclein) antibodies using a dual strategy: i) an amino-terminated self-assembled monolayer activated by glutaraldehyde, and ii) the His-tagged recombinant protein G. In both approaches, comparable sensitivity values were achieved, featuring very low LOD values at the sub-pM level. The microfluidics engineering is central to achieve a controlled functionalisation of the gate electrode and avoid contamination or physisorption on the organic semiconductor. The demonstrated sensing architecture, being a disposable stand-alone chip, can be operated as a point-of-care test, but also it might represent a promising label-free tool to explore in-vitro protein aggregation that takes place during the progression of neurodegenerative illnesses.

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Sensors and Actuators, B: Chemical	Sensors and Actuators, B: Chemical, 3, 5.77% Sensors and Actuators, B: Chemical 3 publications, 5.77%
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Russian Chemical Reviews	Russian Chemical Reviews, 2, 3.85% Russian Chemical Reviews 2 publications, 3.85%
ACS applied materials & interfaces	ACS applied materials & interfaces, 2, 3.85% ACS applied materials & interfaces 2 publications, 3.85%
ACS Applied Electronic Materials	ACS Applied Electronic Materials, 2, 3.85% ACS Applied Electronic Materials 2 publications, 3.85%
Molecules	Molecules, 1, 1.92% Molecules 1 publication, 1.92%
Nature Reviews Methods Primers	Nature Reviews Methods Primers, 1, 1.92% Nature Reviews Methods Primers 1 publication, 1.92%
Food and Chemical Toxicology	Food and Chemical Toxicology, 1, 1.92% Food and Chemical Toxicology 1 publication, 1.92%
Journal of Biotechnology	Journal of Biotechnology, 1, 1.92% Journal of Biotechnology 1 publication, 1.92%
Process Biochemistry	Process Biochemistry, 1, 1.92% Process Biochemistry 1 publication, 1.92%
Advanced Materials Technologies	Advanced Materials Technologies, 1, 1.92% Advanced Materials Technologies 1 publication, 1.92%
Advanced healthcare materials	Advanced healthcare materials, 1, 1.92% Advanced healthcare materials 1 publication, 1.92%
View	View, 1, 1.92% View 1 publication, 1.92%
Advanced Materials Interfaces	Advanced Materials Interfaces, 1, 1.92% Advanced Materials Interfaces 1 publication, 1.92%
Advanced Electronic Materials	Advanced Electronic Materials, 1, 1.92% Advanced Electronic Materials 1 publication, 1.92%
Langmuir	Langmuir, 1, 1.92% Langmuir 1 publication, 1.92%
Nanoscale Advances	Nanoscale Advances, 1, 1.92% Nanoscale Advances 1 publication, 1.92%
Journal of Physical Chemistry C	Journal of Physical Chemistry C, 1, 1.92% Journal of Physical Chemistry C 1 publication, 1.92%
Nano Letters	Nano Letters, 1, 1.92% Nano Letters 1 publication, 1.92%
Chemical Reviews	Chemical Reviews, 1, 1.92% Chemical Reviews 1 publication, 1.92%
CrystEngComm	CrystEngComm, 1, 1.92% CrystEngComm 1 publication, 1.92%
Chemical Communications	Chemical Communications, 1, 1.92% Chemical Communications 1 publication, 1.92%
Journal of Materials Chemistry B	Journal of Materials Chemistry B, 1, 1.92% Journal of Materials Chemistry B 1 publication, 1.92%
Journal of Materials Chemistry C	Journal of Materials Chemistry C, 1, 1.92% Journal of Materials Chemistry C 1 publication, 1.92%
Chemistry of Materials	Chemistry of Materials, 1, 1.92% Chemistry of Materials 1 publication, 1.92%
	1 2 3

Citations by publishers

	2 4 6 8 10 12
Elsevier	Elsevier, 11, 21.15% Elsevier 11 publications, 21.15%
American Chemical Society (ACS)	American Chemical Society (ACS), 10, 19.23% American Chemical Society (ACS) 10 publications, 19.23%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 8, 15.38% Multidisciplinary Digital Publishing Institute (MDPI) 8 publications, 15.38%
Wiley	Wiley, 8, 15.38% Wiley 8 publications, 15.38%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 7, 13.46% Royal Society of Chemistry (RSC) 7 publications, 13.46%
Springer Nature	Springer Nature, 2, 3.85% Springer Nature 2 publications, 3.85%
Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii	Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii, 2, 3.85% Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii 2 publications, 3.85%
IEEE	IEEE, 1, 1.92% IEEE 1 publication, 1.92%
Taylor & Francis	Taylor & Francis, 1, 1.92% Taylor & Francis 1 publication, 1.92%
IOP Publishing	IOP Publishing, 1, 1.92% IOP Publishing 1 publication, 1.92%
	2 4 6 8 10 12

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Ricci S. et al. Label-free immunodetection of α-synuclein by using a microfluidics coplanar electrolyte-gated organic field-effect transistor // Biosensors and Bioelectronics. 2020. Vol. 167. p. 112433.

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Ricci S., Casalini S., Parkula V., Selvaraj M., Saygin G. D., Greco P., Biscarini F., Mas-Torrent M. Label-free immunodetection of α-synuclein by using a microfluidics coplanar electrolyte-gated organic field-effect transistor // Biosensors and Bioelectronics. 2020. Vol. 167. p. 112433.

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RIS Copy

TY - JOUR

DO - 10.1016/j.bios.2020.112433

UR - https://doi.org/10.1016/j.bios.2020.112433

TI - Label-free immunodetection of α-synuclein by using a microfluidics coplanar electrolyte-gated organic field-effect transistor

T2 - Biosensors and Bioelectronics

AU - Ricci, Simona

AU - Casalini, S

AU - Parkula, Vitaliy

AU - Selvaraj, Meenu

AU - Saygin, Gulseren Deniz

AU - Greco, Pierpaolo

AU - Biscarini, F.

AU - Mas-Torrent, Marta

PY - 2020

DA - 2020/11/01 00:00:00

PB - Elsevier

SP - 112433

VL - 167

SN - 0956-5663

SN - 1873-4235

ER -

BibTex

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BibTex Copy

@article{2020_Ricci,

author = {Simona Ricci and S Casalini and Vitaliy Parkula and Meenu Selvaraj and Gulseren Deniz Saygin and Pierpaolo Greco and F. Biscarini and Marta Mas-Torrent},

title = {Label-free immunodetection of α-synuclein by using a microfluidics coplanar electrolyte-gated organic field-effect transistor},

journal = {Biosensors and Bioelectronics},

year = {2020},

volume = {167},

publisher = {Elsevier},

month = {nov},

url = {https://doi.org/10.1016/j.bios.2020.112433},

pages = {112433},

doi = {10.1016/j.bios.2020.112433}

}

Found error?

Publisher

Elsevier

Journal

Biosensors and Bioelectronics

Quartile SCImago

Quartile WOS

Impact factor

12.6

ISSN

09565663 (Print)
18734235 (Electronic)