Biosensors and Bioelectronics, volume 182, pages 113144

Label free detection of miRNA-21 with electrolyte gated organic field effect transistors (EGOFETs)

Meenu Selvaraj ^{1, 2}

Pierpaolo Greco ³

Matteo Sensi ³

Gulseren Deniz Saygin ^{1, 4}

Noemi Bellassai ²

Roberta Dagata ²

Giuseppe Spoto ²

F. Biscarini ^{3, 5}

Hide authors affiliations Show authors affiliations: 5 affiliations

Scriba Nanotecnologie s.r.l., Via di Corticella 183/8, I-40128, Bologna, Italy |

Department of Chemical Sciences, Università degli Studi di Catania, Viale Andrea Doria 6, I-95125, Catania, Italy. |

Department of Life Sciences, Università, Degli Studi di Modena e Reggio Emilia, Via Campi 103, I-41125, Modena, Italy |

⁴

Department of Physics, Informatics and Mathematics, Università, Degli Studi di Modena e Reggio Emilia, Via Campi 103, I-41125, Modena, Italy. |

⁵

Center for Translational Neurophysiology - Istituto Italiano di Tecnologia, Via Fossato di Mortara 17-19, I-44100, Ferrara, Italy. |

Publication type: Journal Article

Publication date: 2021-06-01

Elsevier

Journal: Biosensors and Bioelectronics

Quartile SCImago

Quartile WOS

Impact factor: 12.6

ISSN: 09565663, 18734235

DOI: 10.1016/j.bios.2021.113144

Copy DOI

General Medicine

Biophysics

Electrochemistry

Biotechnology

Biomedical Engineering

Abstract

We report a dual gate/common channel organic transistor architecture designed for quantifying the concentration of one of the strands of miRNA-21 in solution. The device allows one to measure the differential response between two gate electrodes, viz. one sensing and one reference, both immersed in the electrolyte above the transistor channel. Hybridization with oligonucleotide in the picomolar regime induces a sizable reduction of the current flowing through the transistor channel. The device signal is reported at various gate voltages, showing maximum sensitivity in the sublinear regime, with a limit of detection as low as 35 pM. We describe the dose curves with an analytical function derived from a thermodynamic model of the reaction equilibria relevant in our experiment and device configuration, and we show that the apparent Hill dependence on analyte concentration, whose exponent lies between 0.5 and 1, emerges from the interplay of the different equilibria. The binding free energy characteristic of the hybridization on the device surface is found to be approximately 20% lower with respect to the reaction in solution, hinting to partially inhibiting effect of the surface and presence of competing reactions. Impedance spectroscopy and surface plasmon resonance (SPR) performed on the same oligonucleotide pair were correlated to the electronic current transduced by the EGOFET, and confirmed the selectivity of the biorecognition probe covalently bound on the gold surface. • Organic electronics devices operate in solution to detect microRNA. • Picomolar sensitivity is achieved with dual gate electrodes architecture. • Interplay of reaction equilibria explains biosensor signal response. • Surface plasmon resonance validated transduction of the new electronic biosensor.

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Citations by journals

	1 2 3
Advanced Materials	Advanced Materials, 3, 10% Advanced Materials 3 publications, 10%
Advanced Materials Interfaces	Advanced Materials Interfaces, 2, 6.67% Advanced Materials Interfaces 2 publications, 6.67%
Sensors and Actuators, B: Chemical	Sensors and Actuators, B: Chemical, 2, 6.67% Sensors and Actuators, B: Chemical 2 publications, 6.67%
Journal of Materials Chemistry C	Journal of Materials Chemistry C, 2, 6.67% Journal of Materials Chemistry C 2 publications, 6.67%
Micromachines	Micromachines, 1, 3.33% Micromachines 1 publication, 3.33%
Biomicrofluidics	Biomicrofluidics, 1, 3.33% Biomicrofluidics 1 publication, 3.33%
Nature Reviews Methods Primers	Nature Reviews Methods Primers, 1, 3.33% Nature Reviews Methods Primers 1 publication, 3.33%
Bioprinting	Bioprinting, 1, 3.33% Bioprinting 1 publication, 3.33%
Biosensors and Bioelectronics	Biosensors and Bioelectronics, 1, 3.33% Biosensors and Bioelectronics 1 publication, 3.33%
Journal Physics D: Applied Physics	Journal Physics D: Applied Physics, 1, 3.33% Journal Physics D: Applied Physics 1 publication, 3.33%
Neuromorphic Computing and Engineering	Neuromorphic Computing and Engineering, 1, 3.33% Neuromorphic Computing and Engineering 1 publication, 3.33%
Macromolecular Materials and Engineering	Macromolecular Materials and Engineering, 1, 3.33% Macromolecular Materials and Engineering 1 publication, 3.33%
Nano Letters	Nano Letters, 1, 3.33% Nano Letters 1 publication, 3.33%
IEEE Journal on Flexible Electronics	IEEE Journal on Flexible Electronics, 1, 3.33% IEEE Journal on Flexible Electronics 1 publication, 3.33%
Advanced Functional Materials	Advanced Functional Materials, 1, 3.33% Advanced Functional Materials 1 publication, 3.33%
Chemistry of Materials	Chemistry of Materials, 1, 3.33% Chemistry of Materials 1 publication, 3.33%
International Journal of Electrochemical Science	International Journal of Electrochemical Science, 1, 3.33% International Journal of Electrochemical Science 1 publication, 3.33%
The Analyst	The Analyst, 1, 3.33% The Analyst 1 publication, 3.33%
Analytica Chimica Acta	Analytica Chimica Acta, 1, 3.33% Analytica Chimica Acta 1 publication, 3.33%
Advanced Sensor Research	Advanced Sensor Research, 1, 3.33% Advanced Sensor Research 1 publication, 3.33%
Cell Reports Physical Science	Cell Reports Physical Science, 1, 3.33% Cell Reports Physical Science 1 publication, 3.33%
Russian Chemical Reviews	Russian Chemical Reviews, 1, 3.33% Russian Chemical Reviews 1 publication, 3.33%
Angewandte Chemie	Angewandte Chemie, 1, 3.33% Angewandte Chemie 1 publication, 3.33%
Angewandte Chemie - International Edition	Angewandte Chemie - International Edition, 1, 3.33% Angewandte Chemie - International Edition 1 publication, 3.33%
	1 2 3

Citations by publishers

	2 4 6 8 10
Wiley	Wiley, 10, 33.33% Wiley 10 publications, 33.33%
Elsevier	Elsevier, 6, 20% Elsevier 6 publications, 20%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 3, 10% Royal Society of Chemistry (RSC) 3 publications, 10%
IOP Publishing	IOP Publishing, 2, 6.67% IOP Publishing 2 publications, 6.67%
American Chemical Society (ACS)	American Chemical Society (ACS), 2, 6.67% American Chemical Society (ACS) 2 publications, 6.67%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 1, 3.33% Multidisciplinary Digital Publishing Institute (MDPI) 1 publication, 3.33%
American Institute of Physics (AIP)	American Institute of Physics (AIP), 1, 3.33% American Institute of Physics (AIP) 1 publication, 3.33%
Springer Nature	Springer Nature, 1, 3.33% Springer Nature 1 publication, 3.33%
IEEE	IEEE, 1, 3.33% IEEE 1 publication, 3.33%
Electrochemical Science Group, University of Belgrade	Electrochemical Science Group, University of Belgrade, 1, 3.33% Electrochemical Science Group, University of Belgrade 1 publication, 3.33%
Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii	Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii, 1, 3.33% Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii 1 publication, 3.33%
	2 4 6 8 10

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Selvaraj M. et al. Label free detection of miRNA-21 with electrolyte gated organic field effect transistors (EGOFETs) // Biosensors and Bioelectronics. 2021. Vol. 182. p. 113144.

GOST all authors (up to 50) Copy

Selvaraj M., Greco P., Sensi M., Saygin G. D., Bellassai N., Dagata R., Spoto G., Biscarini F. Label free detection of miRNA-21 with electrolyte gated organic field effect transistors (EGOFETs) // Biosensors and Bioelectronics. 2021. Vol. 182. p. 113144.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1016/j.bios.2021.113144

UR - https://doi.org/10.1016/j.bios.2021.113144

TI - Label free detection of miRNA-21 with electrolyte gated organic field effect transistors (EGOFETs)

T2 - Biosensors and Bioelectronics

AU - Selvaraj, Meenu

AU - Greco, Pierpaolo

AU - Sensi, Matteo

AU - Saygin, Gulseren Deniz

AU - Bellassai, Noemi

AU - Dagata, Roberta

AU - Spoto, Giuseppe

AU - Biscarini, F.

PY - 2021

DA - 2021/06/01 00:00:00

PB - Elsevier

SP - 113144

VL - 182

SN - 0956-5663

SN - 1873-4235

ER -

BibTex

Cite this

BibTex Copy

@article{2021_Selvaraj,

author = {Meenu Selvaraj and Pierpaolo Greco and Matteo Sensi and Gulseren Deniz Saygin and Noemi Bellassai and Roberta Dagata and Giuseppe Spoto and F. Biscarini},

title = {Label free detection of miRNA-21 with electrolyte gated organic field effect transistors (EGOFETs)},

journal = {Biosensors and Bioelectronics},

year = {2021},

volume = {182},

publisher = {Elsevier},

month = {jun},

url = {https://doi.org/10.1016/j.bios.2021.113144},

pages = {113144},

doi = {10.1016/j.bios.2021.113144}

}

Found error?

Publisher

Elsevier

Journal

Biosensors and Bioelectronics

Quartile SCImago

Quartile WOS

Impact factor

12.6

ISSN

09565663 (Print)
18734235 (Electronic)