Simultaneous detection of alkaline phosphatase and β-galactosidase activity using SERRS
Тип публикации: Journal Article
Дата публикации: 2009-03-01
scimago Q2
wos Q2
БС2
SJR: 0.472
CiteScore: 5.1
Impact factor: 2.2
ISSN: 0960894X, 14643405
PubMed ID:
19243940
Organic Chemistry
Drug Discovery
Biochemistry
Molecular Biology
Pharmaceutical Science
Clinical Biochemistry
Molecular Medicine
Краткое описание
Surface enhanced resonance Raman scattering (SERRS) is an alternative to fluorescence for use in bioanalysis however due to the different optical mechanism it requires specifically designed reporters. Recently we have reported the use of 8-hydroxyquinolinyl azo dyes and their ester derivatives as reporters of lipase activity using SERRS. Acylation of the 8-hydroxy moiety significantly reduces surface enhancement of the Raman response and subsequent lipase catalysed ester hydrolysis enables the analyte to bind to silver nanoparticles, thus providing surface enhancement and the SERRS signal is 'switched on'. By following this principle, phosphorylated and galactosylated analogues of 8-hydroxyquinolinylazo dyes were prepared and shown to act as reporters of enzymatic activity for alkaline phosphatase and beta-galactosidase respectively when using SERRS.
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Ingram A., Moore B. D., Graham D. Simultaneous detection of alkaline phosphatase and β-galactosidase activity using SERRS // Bioorganic and Medicinal Chemistry Letters. 2009. Vol. 19. No. 6. pp. 1569-1571.
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Ingram A., Moore B. D., Graham D. Simultaneous detection of alkaline phosphatase and β-galactosidase activity using SERRS // Bioorganic and Medicinal Chemistry Letters. 2009. Vol. 19. No. 6. pp. 1569-1571.
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TY - JOUR
DO - 10.1016/j.bmcl.2009.02.030
UR - https://doi.org/10.1016/j.bmcl.2009.02.030
TI - Simultaneous detection of alkaline phosphatase and β-galactosidase activity using SERRS
T2 - Bioorganic and Medicinal Chemistry Letters
AU - Ingram, Andy
AU - Moore, Barry D
AU - Graham, Duncan
PY - 2009
DA - 2009/03/01
PB - Elsevier
SP - 1569-1571
IS - 6
VL - 19
PMID - 19243940
SN - 0960-894X
SN - 1464-3405
ER -
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BibTex (до 50 авторов)
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@article{2009_Ingram,
author = {Andy Ingram and Barry D Moore and Duncan Graham},
title = {Simultaneous detection of alkaline phosphatase and β-galactosidase activity using SERRS},
journal = {Bioorganic and Medicinal Chemistry Letters},
year = {2009},
volume = {19},
publisher = {Elsevier},
month = {mar},
url = {https://doi.org/10.1016/j.bmcl.2009.02.030},
number = {6},
pages = {1569--1571},
doi = {10.1016/j.bmcl.2009.02.030}
}
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MLA
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Ingram, Andy, et al. “Simultaneous detection of alkaline phosphatase and β-galactosidase activity using SERRS.” Bioorganic and Medicinal Chemistry Letters, vol. 19, no. 6, Mar. 2009, pp. 1569-1571. https://doi.org/10.1016/j.bmcl.2009.02.030.