Cytotoxicity determination without photochemical artifacts
Тип публикации: Journal Article
Дата публикации: 2005-06-01
scimago Q1
wos Q1
БС1
SJR: 2.756
CiteScore: 13.9
Impact factor: 10.1
ISSN: 03043835, 18727980
PubMed ID:
15890237
Cancer Research
Oncology
Краткое описание
A study was performed to improve cytotoxicity determinations by eliminating flavin-mediated photosensitization from tests with KB cells, NCI-H69 cells, P-glycoprotein expressing KBC5-8 cells, MRP1-expressing H69AR cells, and A240286S human lung adenocarcinoma cells. Growth inhibition by cis-platin, doxorubicin, etoposide, gemcitabine, taxol, vincristine, vinblastine, and vinorelbine was determined under flavin-protecting conditions using flavin-free culture media with fetal bovine serum as the only source of flavins. As compared to conventional tests, the IC50 values determined under flavin-protecting conditions reflected increased apparent drug cytotoxicities, and were flawlessly reproducible. Flavin-mediated photosensitization should, therefore, be strictly eliminated from in vitro experiments involving cytotoxic and other drugs.
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ГОСТ
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Heuser M. et al. Cytotoxicity determination without photochemical artifacts // Cancer Letters. 2005. Vol. 223. No. 1. pp. 57-66.
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Heuser M., Kopun M., Rittgen W., Granzow C. Cytotoxicity determination without photochemical artifacts // Cancer Letters. 2005. Vol. 223. No. 1. pp. 57-66.
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TY - JOUR
DO - 10.1016/j.canlet.2004.10.031
UR - https://doi.org/10.1016/j.canlet.2004.10.031
TI - Cytotoxicity determination without photochemical artifacts
T2 - Cancer Letters
AU - Heuser, M.
AU - Kopun, Marijana
AU - Rittgen, Werner
AU - Granzow, Christof
PY - 2005
DA - 2005/06/01
PB - Elsevier
SP - 57-66
IS - 1
VL - 223
PMID - 15890237
SN - 0304-3835
SN - 1872-7980
ER -
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@article{2005_Heuser,
author = {M. Heuser and Marijana Kopun and Werner Rittgen and Christof Granzow},
title = {Cytotoxicity determination without photochemical artifacts},
journal = {Cancer Letters},
year = {2005},
volume = {223},
publisher = {Elsevier},
month = {jun},
url = {https://doi.org/10.1016/j.canlet.2004.10.031},
number = {1},
pages = {57--66},
doi = {10.1016/j.canlet.2004.10.031}
}
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MLA
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Heuser, M., et al. “Cytotoxicity determination without photochemical artifacts.” Cancer Letters, vol. 223, no. 1, Jun. 2005, pp. 57-66. https://doi.org/10.1016/j.canlet.2004.10.031.