Open Access

Cell, volume 153, issue 4, pages 910-918

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

Wang Hao-yi ¹

Yang Hui ¹

Shivalila Chikdu S ²

Dawlaty Meelad M ¹

Cheng Albert ³

Zhang Feng ^{4, 5}

Jaenisch Rudolf ³

Hide authors affiliations Show authors affiliations: 5 affiliations

Whitehead Institute for Biomedical Research, Cambridge, MA 02142 (USA). |

Whitehead Institute for Biomedical Research, Cambridge, MA, 02142, USA. |

Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA. |

⁴

Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA |

⁵

McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA |

Publication type: Journal Article

Publication date: 2013-05-02

Elsevier

Journal: Cell

Quartile SCImago

Quartile WOS

Impact factor: 64.5

ISSN: 00928674, 10974172

DOI: 10.1016/j.cell.2013.04.025

Copy DOI

PubMed ID: 23643243

General Biochemistry, Genetics and Molecular Biology

Abstract

Mice carrying mutations in multiple genes are traditionally generated by sequential recombination in embryonic stem cells and/or time-consuming intercrossing of mice with a single mutation. The CRISPR/Cas system has been adapted as an efficient gene-targeting technology with the potential for multiplexed genome editing. We demonstrate that CRISPR/Cas-mediated gene editing allows the simultaneous disruption of five genes (Tet1, 2, 3, Sry, Uty--8 alleles) in mouse embryonic stem (ES) cells with high efficiency. Coinjection of Cas9 mRNA and single-guide RNAs (sgRNAs) targeting Tet1 and Tet2 into zygotes generated mice with biallelic mutations in both genes with an efficiency of 80%. Finally, we show that coinjection of Cas9 mRNA/sgRNAs with mutant oligos generated precise point mutations simultaneously in two target genes. Thus, the CRISPR/Cas system allows the one-step generation of animals carrying mutations in multiple genes, an approach that will greatly accelerate the in vivo study of functionally redundant genes and of epistatic gene interactions.

By date By citations

Citations by journals

	20 40 60 80 100 120 140
Scientific Reports	Scientific Reports, 136, 4.83% Scientific Reports 136 publications, 4.83%
Methods in Molecular Biology	Methods in Molecular Biology, 87, 3.09% Methods in Molecular Biology 87 publications, 3.09%
PLoS ONE	PLoS ONE, 69, 2.45% PLoS ONE 69 publications, 2.45%
Nature Communications	Nature Communications, 62, 2.2% Nature Communications 62 publications, 2.2%
Nucleic Acids Research	Nucleic Acids Research, 37, 1.31% Nucleic Acids Research 37 publications, 1.31%
Proceedings of the National Academy of Sciences of the United States of America	Proceedings of the National Academy of Sciences of the United States of America, 33, 1.17% Proceedings of the National Academy of Sciences of the United States of America 33 publications, 1.17%
Cell Reports	Cell Reports, 32, 1.14% Cell Reports 32 publications, 1.14%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 29, 1.03% International Journal of Molecular Sciences 29 publications, 1.03%
Nature	Nature, 27, 0.96% Nature 27 publications, 0.96%
Transgenic Research	Transgenic Research, 25, 0.89% Transgenic Research 25 publications, 0.89%
Cell	Cell, 25, 0.89% Cell 25 publications, 0.89%
eLife	eLife, 24, 0.85% eLife 24 publications, 0.85%
Development (Cambridge)	Development (Cambridge), 23, 0.82% Development (Cambridge) 23 publications, 0.82%
Nature Biotechnology	Nature Biotechnology, 23, 0.82% Nature Biotechnology 23 publications, 0.82%
Journal of Genetics and Genomics	Journal of Genetics and Genomics, 22, 0.78% Journal of Genetics and Genomics 22 publications, 0.78%
Journal of Biological Chemistry	Journal of Biological Chemistry, 18, 0.64% Journal of Biological Chemistry 18 publications, 0.64%
Nature Protocols	Nature Protocols, 17, 0.6% Nature Protocols 17 publications, 0.6%
Biochemical and Biophysical Research Communications	Biochemical and Biophysical Research Communications, 16, 0.57% Biochemical and Biophysical Research Communications 16 publications, 0.57%
Advances in Experimental Medicine and Biology	Advances in Experimental Medicine and Biology, 15, 0.53% Advances in Experimental Medicine and Biology 15 publications, 0.53%
Genetics	Genetics, 15, 0.53% Genetics 15 publications, 0.53%
Methods	Methods, 14, 0.5% Methods 14 publications, 0.5%
Mammalian Genome	Mammalian Genome, 13, 0.46% Mammalian Genome 13 publications, 0.46%
Cell Research	Cell Research, 13, 0.46% Cell Research 13 publications, 0.46%
Science	Science, 13, 0.46% Science 13 publications, 0.46%
Human Molecular Genetics	Human Molecular Genetics, 13, 0.46% Human Molecular Genetics 13 publications, 0.46%
Biology of Reproduction	Biology of Reproduction, 13, 0.46% Biology of Reproduction 13 publications, 0.46%
Developmental Biology	Developmental Biology, 12, 0.43% Developmental Biology 12 publications, 0.43%
Journal of Reproduction and Development	Journal of Reproduction and Development, 12, 0.43% Journal of Reproduction and Development 12 publications, 0.43%
BMC Biotechnology	BMC Biotechnology, 11, 0.39% BMC Biotechnology 11 publications, 0.39%
	20 40 60 80 100 120 140

Citations by publishers

	100 200 300 400 500 600 700 800
Springer Nature	Springer Nature, 780, 27.72% Springer Nature 780 publications, 27.72%
Elsevier	Elsevier, 523, 18.59% Elsevier 523 publications, 18.59%
Wiley	Wiley, 219, 7.78% Wiley 219 publications, 7.78%
Oxford University Press	Oxford University Press, 105, 3.73% Oxford University Press 105 publications, 3.73%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 88, 3.13% Public Library of Science (PLoS) 88 publications, 3.13%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 83, 2.95% Multidisciplinary Digital Publishing Institute (MDPI) 83 publications, 2.95%
Frontiers Media S.A.	Frontiers Media S.A., 68, 2.42% Frontiers Media S.A. 68 publications, 2.42%
Taylor & Francis	Taylor & Francis, 44, 1.56% Taylor & Francis 44 publications, 1.56%
The Company of Biologists	The Company of Biologists, 43, 1.53% The Company of Biologists 43 publications, 1.53%
Proceedings of the National Academy of Sciences (PNAS)	Proceedings of the National Academy of Sciences (PNAS), 33, 1.17% Proceedings of the National Academy of Sciences (PNAS) 33 publications, 1.17%
Cold Spring Harbor Laboratory	Cold Spring Harbor Laboratory, 33, 1.17% Cold Spring Harbor Laboratory 33 publications, 1.17%
Wolters Kluwer Health	Wolters Kluwer Health, 25, 0.89% Wolters Kluwer Health 25 publications, 0.89%
American Association for the Advancement of Science (AAAS)	American Association for the Advancement of Science (AAAS), 25, 0.89% American Association for the Advancement of Science (AAAS) 25 publications, 0.89%
Genetics Society of America	Genetics Society of America, 24, 0.85% Genetics Society of America 24 publications, 0.85%
eLife Sciences Publications	eLife Sciences Publications, 24, 0.85% eLife Sciences Publications 24 publications, 0.85%
American Chemical Society (ACS)	American Chemical Society (ACS), 23, 0.82% American Chemical Society (ACS) 23 publications, 0.82%
American Society for Biochemistry and Molecular Biology	American Society for Biochemistry and Molecular Biology, 22, 0.78% American Society for Biochemistry and Molecular Biology 22 publications, 0.78%
SAGE	SAGE, 21, 0.75% SAGE 21 publications, 0.75%
Mary Ann Liebert	Mary Ann Liebert, 19, 0.68% Mary Ann Liebert 19 publications, 0.68%
EMBO press	EMBO press, 17, 0.6% EMBO press 17 publications, 0.6%
American Society for Microbiology	American Society for Microbiology, 17, 0.6% American Society for Microbiology 17 publications, 0.6%
Annual Reviews	Annual Reviews, 15, 0.53% Annual Reviews 15 publications, 0.53%
Society for the Study of Reproduction	Society for the Study of Reproduction, 13, 0.46% Society for the Study of Reproduction 13 publications, 0.46%
IPEC Inc.	IPEC Inc., 12, 0.43% IPEC Inc. 12 publications, 0.43%
Hindawi Limited	Hindawi Limited, 11, 0.39% Hindawi Limited 11 publications, 0.39%
Rockefeller University Press	Rockefeller University Press, 11, 0.39% Rockefeller University Press 11 publications, 0.39%
American Society for Clinical Investigation	American Society for Clinical Investigation, 9, 0.32% American Society for Clinical Investigation 9 publications, 0.32%
Pleiades Publishing	Pleiades Publishing, 8, 0.28% Pleiades Publishing 8 publications, 0.28%
Impact Journals	Impact Journals, 8, 0.28% Impact Journals 8 publications, 0.28%
	100 200 300 400 500 600 700 800

We do not take into account publications that without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
Statistics recalculated weekly.

{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2013,2014,2015,2016,2017,2018,2019,2020,2021,2022,2023,2024],"ids":[0,0,0,0,0,0,0,0,0,0,0,0],"codes":[0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["","","","","","","","","","","",""],"datasets":[{"label":"Citations number","data":[109,310,300,346,316,292,268,248,218,192,181,34],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["3.87","11.02","10.66","12.3","11.23","10.38","9.52","8.81","7.75","6.82","6.43","1.21"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Scientific Reports","Methods in Molecular Biology","PLoS ONE","Nature Communications","Nucleic Acids Research","Proceedings of the National Academy of Sciences of the United States of America","Cell Reports","International Journal of Molecular Sciences","Nature","Transgenic Research","Cell","eLife","Development (Cambridge)","Nature Biotechnology","Journal of Genetics and Genomics","Journal of Biological Chemistry","Nature Protocols","Biochemical and Biophysical Research Communications","Advances in Experimental Medicine and Biology","Genetics","Methods","Mammalian Genome","Cell Research","Science","Human Molecular Genetics","Biology of Reproduction","Developmental Biology","Journal of Reproduction and Development","BMC Biotechnology"],"ids":[13767,3516,24963,3231,23904,306,9130,14627,14611,1313,16552,24138,2238,18127,7249,19451,21812,12390,17590,10675,16671,3173,10892,7711,4947,7809,3763,5691,8791],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/mxFdPe9qujsfvfYfcN0QOclAiYORFb0xrRlwV8gs_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/eIZgkq1MRZyJ3hBkdupnUUv7wgB6zCl9D99SlPN5_medium.webp","\/storage\/images\/resized\/ko5EaisKGm66J1hHXtfEgVEeeWb9uOcMofpNuf0a_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/OuA6WPZekwEiUM99mP2pSqj4yHPxHezM6xgLhAjO_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/9EVpRifnnUJt6oHGY5GfsfSXTEz5wzbdgnZqiF5m_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/s10mcsCV4OAUg9O2KrqOquQC0PhyLMI8hUUkuflM_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/OQ7TJyrdS1q8HnKdPSYhEebWzI7q5LxLYiQeWuua_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp"],"datasets":[{"label":"","data":[136,87,69,62,37,33,32,29,27,25,25,24,23,23,22,18,17,16,15,15,14,13,13,13,13,13,12,12,11],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[4.83,3.09,2.45,2.2,1.31,1.17,1.14,1.03,0.96,0.89,0.89,0.85,0.82,0.82,0.78,0.64,0.6,0.57,0.53,0.53,0.5,0.46,0.46,0.46,0.46,0.46,0.43,0.43,0.39],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Springer Nature","Elsevier","Wiley","Oxford University Press","Public Library of Science (PLoS)","Multidisciplinary Digital Publishing Institute (MDPI)","Frontiers Media S.A.","Taylor & Francis","The Company of Biologists","Proceedings of the National Academy of Sciences (PNAS)","Cold Spring Harbor Laboratory","Wolters Kluwer Health","American Association for the Advancement of Science (AAAS)","Genetics Society of America","eLife Sciences Publications","American Chemical Society (ACS)","American Society for Biochemistry and Molecular Biology","SAGE","Mary Ann Liebert","EMBO press","American Society for Microbiology","Annual Reviews","Society for the Study of Reproduction","IPEC Inc.","Hindawi Limited","Rockefeller University Press","American Society for Clinical Investigation","Pleiades Publishing","Impact Journals"],"ids":[8,17,11,19,344,202,208,18,872,162,6909,32,189,3356,6627,40,2090,31,230,9415,142,6941,2584,1973,6921,22,564,101,342],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/ko5EaisKGm66J1hHXtfEgVEeeWb9uOcMofpNuf0a_medium.webp","\/storage\/images\/resized\/mxFdPe9qujsfvfYfcN0QOclAiYORFb0xrRlwV8gs_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/6QE8LXWrLpkoy5A2te6hw7who46GeCoTYIstuoAz_medium.webp","\/storage\/images\/resized\/s10mcsCV4OAUg9O2KrqOquQC0PhyLMI8hUUkuflM_medium.webp","\/storage\/images\/resized\/9EVpRifnnUJt6oHGY5GfsfSXTEz5wzbdgnZqiF5m_medium.webp","\/storage\/images\/resized\/eIZgkq1MRZyJ3hBkdupnUUv7wgB6zCl9D99SlPN5_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/OuA6WPZekwEiUM99mP2pSqj4yHPxHezM6xgLhAjO_medium.webp","\/storage\/images\/resized\/ruydfaB80LDjlkYqsfOeUAZohOIODyq7bQzis5O7_medium.webp","\/storage\/images\/resized\/Zy5YST233rzRbZP24I24MzZm64hz20ZV9qQopmMn_medium.webp","\/storage\/images\/resized\/1UiI5HycPsvulDeXOySNp0OuaMMmIKfPnsdKPyH0_medium.webp","\/storage\/images\/resized\/VPE7q24PXoHr5SS2SqfEgxccSMUxWLfZINOX60uo_medium.webp","\/storage\/images\/resized\/A7oGMwx1m3fFJlKJvQUVra8h0itmEa7JZW0q3Q0b_medium.webp","\/storage\/images\/resized\/OQ7TJyrdS1q8HnKdPSYhEebWzI7q5LxLYiQeWuua_medium.webp","","\/storage\/images\/resized\/hqfzhQAjTGlNSRs6yzFNITgjSMm9Jr2QuotJHIvE_medium.webp","\/storage\/images\/resized\/lEA1FyUnFAZR1DchuczZi5bjAixG3TNeWVz6oF4R_medium.webp","\/storage\/images\/resized\/aFa86lAy63Pq5tYKiOsH017UsgJA6uk6ed66C0Ns_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/HY0CXgRt044tzIAB16PDvDzdHeKHGtSvlMH0yWf6_medium.webp"],"datasets":[{"label":"","data":[780,523,219,105,88,83,68,44,43,33,33,25,25,24,24,23,22,21,19,17,17,15,13,12,11,11,9,8,8],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[27.72,18.59,7.78,3.73,3.13,2.95,2.42,1.56,1.53,1.17,1.17,0.89,0.89,0.85,0.85,0.82,0.78,0.75,0.68,0.6,0.6,0.53,0.46,0.43,0.39,0.39,0.32,0.28,0.28],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}

Metrics

Cite this

GOST |

Cite this

GOST Copy

Wang H. et al. One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering // Cell. 2013. Vol. 153. No. 4. pp. 910-918.

GOST all authors (up to 50) Copy

Wang H., Yang H., Shivalila C. S., Dawlaty M. M., Cheng A., Zhang F., Jaenisch R. One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering // Cell. 2013. Vol. 153. No. 4. pp. 910-918.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1016/j.cell.2013.04.025

UR - https://doi.org/10.1016%2Fj.cell.2013.04.025

TI - One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

T2 - Cell

AU - Wang, Hao-yi

AU - Yang, Hui

AU - Shivalila, Chikdu S

AU - Dawlaty, Meelad M

AU - Cheng, Albert

AU - Zhang, Feng

AU - Jaenisch, Rudolf

PY - 2013

DA - 2013/05/02 00:00:00

PB - Elsevier

SP - 910-918

IS - 4

VL - 153

PMID - 23643243

SN - 0092-8674

SN - 1097-4172

ER -

BibTex |

Cite this

BibTex Copy

@article{2013_Wang,

author = {Hao-yi Wang and Hui Yang and Chikdu S Shivalila and Meelad M Dawlaty and Albert Cheng and Feng Zhang and Rudolf Jaenisch},

title = {One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering},

journal = {Cell},

year = {2013},

volume = {153},

publisher = {Elsevier},

month = {may},

url = {https://doi.org/10.1016%2Fj.cell.2013.04.025},

number = {4},

pages = {910--918},

doi = {10.1016/j.cell.2013.04.025}

}

MLA

Cite this

MLA Copy

Wang, Hao-yi, et al. “One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering.” Cell, vol. 153, no. 4, May. 2013, pp. 910-918. https://doi.org/10.1016%2Fj.cell.2013.04.025.

Found error?

Publisher

Elsevier

Journal

Cell

Quartile SCImago

Quartile WOS

Impact factor

64.5

ISSN

00928674 (Print)
10974172 (Electronic)