Open Access

Cell Reports, volume 18, issue 11, pages 2635-2650

Mutually Exclusive CBC-Containing Complexes Contribute to RNA Fate

Giacometti Simone ^{1, 2, 3}

Benbahouche Nour El Houda ⁴

Domanski Michal ⁵

ROBERT MARIE F. ⁴

Meola N. ⁵

Lubas Michal ⁵

Bukenborg Jakob ⁶

Andersen Jens E. T. ⁶

Schulze Wiebke M ⁷

Verheggen Céline ⁴

Kudla Grzegorz ²

Jensen Torben René ⁵

Bertrand E. ⁴

Hide authors affiliations Show authors affiliations: 7 affiliations

Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, Bldg. 1130, 8000 Aarhus C, Denmark |

MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4, UK |

Unité Mixte de Recherche 5535, Institut de Génétique Moléculaire de Montpellier, CNRS and Montpellier University, 34293 Montpellier, France |

⁴

Unité Mixte de Recherche 5535, Institut de Génétique Moléculaire de Montpellier, CNRS and Montpellier University, 34293 Montpellier, France. |

⁵

Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, Bldg. 1130, 8000 Aarhus C, Denmark. |

⁶

§Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark, |

⁷

Grenoble Outstation, European Molecular Biology Laboratory, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble, France |

Publication type: Journal Article

Publication date: 2017-03-14

Elsevier

Journal: Cell Reports

Quartile SCImago

Quartile WOS

Impact factor: 8.8

ISSN: 22111247

DOI: 10.1016/j.celrep.2017.02.046

Copy DOI

PubMed ID: 28297668

General Biochemistry, Genetics and Molecular Biology

Abstract

The nuclear cap-binding complex (CBC) stimulates processing reactions of capped RNAs, including their splicing, 3'-end formation, degradation, and transport. CBC effects are particular for individual RNA families, but how such selectivity is achieved remains elusive. Here, we analyze three main CBC partners known to impact different RNA species. ARS2 stimulates 3'-end formation/transcription termination of several transcript types, ZC3H18 stimulates degradation of a diverse set of RNAs, and PHAX functions in pre-small nuclear RNA/small nucleolar RNA (pre-snRNA/snoRNA) transport. Surprisingly, these proteins all bind capped RNAs without strong preferences for given transcripts, and their steady-state binding correlates poorly with their function. Despite this, PHAX and ZC3H18 compete for CBC binding and we demonstrate that this competitive binding is functionally relevant. We further show that CBC-containing complexes are short lived in vivo, and we therefore suggest that RNA fate involves the transient formation of mutually exclusive CBC complexes, which may only be consequential at particular checkpoints during RNA biogenesis.

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Genes and Development	Genes and Development, 2, 3.03% Genes and Development 2 publications, 3.03%
Development (Cambridge)	Development (Cambridge), 1, 1.52% Development (Cambridge) 1 publication, 1.52%
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International Journal of Molecular Sciences	International Journal of Molecular Sciences, 1, 1.52% International Journal of Molecular Sciences 1 publication, 1.52%
Frontiers in Microbiology	Frontiers in Microbiology, 1, 1.52% Frontiers in Microbiology 1 publication, 1.52%
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Chemical Reviews	Chemical Reviews, 1, 1.52% Chemical Reviews 1 publication, 1.52%
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Plant Cell	Plant Cell, 1, 1.52% Plant Cell 1 publication, 1.52%
Journal of Virology	Journal of Virology, 1, 1.52% Journal of Virology 1 publication, 1.52%
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RNA	RNA, 1, 1.52% RNA 1 publication, 1.52%
Cold Spring Harbor Symposia on Quantitative Biology	Cold Spring Harbor Symposia on Quantitative Biology, 1, 1.52% Cold Spring Harbor Symposia on Quantitative Biology 1 publication, 1.52%
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Citations by publishers

	2 4 6 8 10 12 14
Elsevier	Elsevier, 13, 19.7% Elsevier 13 publications, 19.7%
Oxford University Press	Oxford University Press, 12, 18.18% Oxford University Press 12 publications, 18.18%
Springer Nature	Springer Nature, 11, 16.67% Springer Nature 11 publications, 16.67%
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Frontiers Media S.A.	Frontiers Media S.A., 1, 1.52% Frontiers Media S.A. 1 publication, 1.52%
Wiley	Wiley, 1, 1.52% Wiley 1 publication, 1.52%
American Chemical Society (ACS)	American Chemical Society (ACS), 1, 1.52% American Chemical Society (ACS) 1 publication, 1.52%
EMBO press	EMBO press, 1, 1.52% EMBO press 1 publication, 1.52%
American Society of Plant Biologists	American Society of Plant Biologists, 1, 1.52% American Society of Plant Biologists 1 publication, 1.52%
American Society for Microbiology	American Society for Microbiology, 1, 1.52% American Society for Microbiology 1 publication, 1.52%
	2 4 6 8 10 12 14

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GOST Copy

Giacometti S. et al. Mutually Exclusive CBC-Containing Complexes Contribute to RNA Fate // Cell Reports. 2017. Vol. 18. No. 11. pp. 2635-2650.

GOST all authors (up to 50) Copy

Giacometti S., Benbahouche N. E. H., Domanski M., ROBERT M. F., Meola N., Lubas M., Bukenborg J., Andersen J. E. T., Schulze W. M., Verheggen C., Kudla G., Jensen T. R., Bertrand E. Mutually Exclusive CBC-Containing Complexes Contribute to RNA Fate // Cell Reports. 2017. Vol. 18. No. 11. pp. 2635-2650.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1016/j.celrep.2017.02.046

UR - https://doi.org/10.1016%2Fj.celrep.2017.02.046

TI - Mutually Exclusive CBC-Containing Complexes Contribute to RNA Fate

T2 - Cell Reports

AU - Giacometti, Simone

AU - Benbahouche, Nour El Houda

AU - Domanski, Michal

AU - ROBERT, MARIE F.

AU - Meola, N.

AU - Lubas, Michal

AU - Bukenborg, Jakob

AU - Andersen, Jens E. T.

AU - Schulze, Wiebke M

AU - Verheggen, Céline

AU - Kudla, Grzegorz

AU - Jensen, Torben René

AU - Bertrand, E.

PY - 2017

DA - 2017/03/14 00:00:00

PB - Elsevier

SP - 2635-2650

IS - 11

VL - 18

PMID - 28297668

SN - 2211-1247

ER -

BibTex |

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BibTex Copy

@article{2017_Giacometti,

author = {Simone Giacometti and Nour El Houda Benbahouche and Michal Domanski and MARIE F. ROBERT and N. Meola and Michal Lubas and Jakob Bukenborg and Jens E. T. Andersen and Wiebke M Schulze and Céline Verheggen and Grzegorz Kudla and Torben René Jensen and E. Bertrand},

title = {Mutually Exclusive CBC-Containing Complexes Contribute to RNA Fate},

journal = {Cell Reports},

year = {2017},

volume = {18},

publisher = {Elsevier},

month = {mar},

url = {https://doi.org/10.1016%2Fj.celrep.2017.02.046},

number = {11},

pages = {2635--2650},

doi = {10.1016/j.celrep.2017.02.046}

}

MLA

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MLA Copy

Giacometti, Simone, et al. “Mutually Exclusive CBC-Containing Complexes Contribute to RNA Fate.” Cell Reports, vol. 18, no. 11, Mar. 2017, pp. 2635-2650. https://doi.org/10.1016%2Fj.celrep.2017.02.046.

Found error?

Publisher

Elsevier

Journal

Cell Reports

Quartile SCImago

Quartile WOS

Impact factor

8.8

ISSN

22111247 (Print)