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Cell Reports, volume 23, issue 7, pages 2199-2210

The RNA Exosome Adaptor ZFC3H1 Functionally Competes with Nuclear Export Activity to Retain Target Transcripts

Silla Toomas ¹

Karadoulama Evdoxia

Makosa Dawid ¹

Lubas Michal ¹

Jensen Torben Heick ¹

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Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, building 1130, 8000 Aarhus C, Denmark |

Publication type: Journal Article

Publication date: 2018-05-15

Elsevier

Journal: Cell Reports

Quartile SCImago

Quartile WOS

Impact factor: 8.8

ISSN: 22111247

DOI: 10.1016/j.celrep.2018.04.061

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PubMed ID: 29768216

General Biochemistry, Genetics and Molecular Biology

Abstract

Mammalian genomes are promiscuously transcribed, yielding protein-coding and non-coding products. Many transcripts are short lived due to their nuclear degradation by the ribonucleolytic RNA exosome. Here, we show that abolished nuclear exosome function causes the formation of distinct nuclear foci, containing polyadenylated (pA+) RNA secluded from nucleocytoplasmic export. We asked whether exosome co-factors could serve such nuclear retention. Co-localization studies revealed the enrichment of pA+ RNA foci with "pA-tail exosome targeting (PAXT) connection" components MTR4, ZFC3H1, and PABPN1 but no overlap with known nuclear structures such as Cajal bodies, speckles, paraspeckles, or nucleoli. Interestingly, ZFC3H1 is required for foci formation, and in its absence, selected pA+ RNAs, including coding and non-coding transcripts, are exported to the cytoplasm in a process dependent on the mRNA export factor AlyREF. Our results establish ZFC3H1 as a central nuclear pA+ RNA retention factor, counteracting nuclear export activity.

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Citations by publishers

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Rockefeller University Press	Rockefeller University Press, 2, 3.28% Rockefeller University Press 2 publications, 3.28%
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Public Library of Science (PLoS)	Public Library of Science (PLoS), 1, 1.64% Public Library of Science (PLoS) 1 publication, 1.64%
Wiley	Wiley, 1, 1.64% Wiley 1 publication, 1.64%
American Society of Plant Biologists	American Society of Plant Biologists, 1, 1.64% American Society of Plant Biologists 1 publication, 1.64%
Proceedings of the National Academy of Sciences (PNAS)	Proceedings of the National Academy of Sciences (PNAS), 1, 1.64% Proceedings of the National Academy of Sciences (PNAS) 1 publication, 1.64%
	2 4 6 8 10

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GOST Copy

Silla T. et al. The RNA Exosome Adaptor ZFC3H1 Functionally Competes with Nuclear Export Activity to Retain Target Transcripts // Cell Reports. 2018. Vol. 23. No. 7. pp. 2199-2210.

GOST all authors (up to 50) Copy

Silla T., Karadoulama E., Makosa D., Lubas M., Jensen T. H. The RNA Exosome Adaptor ZFC3H1 Functionally Competes with Nuclear Export Activity to Retain Target Transcripts // Cell Reports. 2018. Vol. 23. No. 7. pp. 2199-2210.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1016/j.celrep.2018.04.061

UR - https://doi.org/10.1016%2Fj.celrep.2018.04.061

TI - The RNA Exosome Adaptor ZFC3H1 Functionally Competes with Nuclear Export Activity to Retain Target Transcripts

T2 - Cell Reports

AU - Silla, Toomas

AU - Karadoulama, Evdoxia

AU - Makosa, Dawid

AU - Lubas, Michal

AU - Jensen, Torben Heick

PY - 2018

DA - 2018/05/15 00:00:00

PB - Elsevier

SP - 2199-2210

IS - 7

VL - 23

PMID - 29768216

SN - 2211-1247

ER -

BibTex |

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BibTex Copy

@article{2018_Silla,

author = {Toomas Silla and Evdoxia Karadoulama and Dawid Makosa and Michal Lubas and Torben Heick Jensen},

title = {The RNA Exosome Adaptor ZFC3H1 Functionally Competes with Nuclear Export Activity to Retain Target Transcripts},

journal = {Cell Reports},

year = {2018},

volume = {23},

publisher = {Elsevier},

month = {may},

url = {https://doi.org/10.1016%2Fj.celrep.2018.04.061},

number = {7},

pages = {2199--2210},

doi = {10.1016/j.celrep.2018.04.061}

}

MLA

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MLA Copy

Silla, Toomas, et al. “The RNA Exosome Adaptor ZFC3H1 Functionally Competes with Nuclear Export Activity to Retain Target Transcripts.” Cell Reports, vol. 23, no. 7, May. 2018, pp. 2199-2210. https://doi.org/10.1016%2Fj.celrep.2018.04.061.

Found error?

Publisher

Elsevier

Journal

Cell Reports

Quartile SCImago

Quartile WOS

Impact factor

8.8

ISSN

22111247 (Print)