Journal of Chromatography A, volume 1504, pages 83-90
Twins labeling-liquid chromatography/mass spectrometry based metabolomics for absolute quantification of tryptophan and its key metabolites
2
3
Publication type: Journal Article
Publication date: 2017-06-01
Journal:
Journal of Chromatography A
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 4.1
ISSN: 00219673, 18733778
Organic Chemistry
Biochemistry
General Medicine
Analytical Chemistry
Abstract
Tryptophan metabolism plays a crucial role in mediating gastrointestinal function. Here, in order to absolutely quantify tryptophan and its metabolites, a liquid chromatography-mass spectrometry (LC-MS) based targeted metabolomics approach was developed using N-dimethyl-/N-diethyl-amino naphthalene-1-sulfonyl chloride (Dns/Dens-Cl) as twins labeling (TL) reagents. Dns-Cl is famous in amine and phenol derivations, and structure is similar with Dens-Cl. The introduction of easily protonated moiety of tertiary ammonium-containing part in the derivatives from Dns to tryptophan and its metabolites not only improved the LC separation but also enhanced their MS response. In addition, the Dens labeled standards were used as internal standards to compensate for matrix effects and ensure accurate quantifications. With the proposed method, twelve metabolites in tryptophan pathway could be detected at sub-ng/mL levels using only 20μL rat serum (the limit of detection could reach 3pg/mL for tryptamine, N-acetyl-serotonin and 6-hydroxymelatonin). The sensitivity was enhanced about 1-2 orders of magnitude compared with non-derivatization method. Focusing on tryptophan pathway, the method was successfully applied to determine the absolute serum concentrations of twelve tryptophan metabolites in a vincristine-induced ileus rat model. A significant down-regulation of the tryptophan metabolism along the kynurenine pathway and up-regulation of serotonin pathway were uncovered. Our findings provide a deeper insight into the mechanism of gastrointestinal dysfunction.
Top-30
Citations by journals
|
1
2
3
|
|
|
Journal of Chromatography A
3 publications, 13.04%
|
|
|
Analytica Chimica Acta
2 publications, 8.7%
|
|
|
Bioanalysis
1 publication, 4.35%
|
|
|
Journal of Analysis and Testing
1 publication, 4.35%
|
|
|
Journal of Pharmaceutical and Biomedical Analysis
1 publication, 4.35%
|
|
|
Chinese Chemical Letters
1 publication, 4.35%
|
|
|
Forensic Science International: Synergy
1 publication, 4.35%
|
|
|
TrAC - Trends in Analytical Chemistry
1 publication, 4.35%
|
|
|
Microchemical Journal
1 publication, 4.35%
|
|
|
Acta Pharmaceutica Sinica B
1 publication, 4.35%
|
|
|
Talanta
1 publication, 4.35%
|
|
|
Journal of Neurochemistry
1 publication, 4.35%
|
|
|
Chemical Research in Toxicology
1 publication, 4.35%
|
|
|
Journal of Dairy Science
1 publication, 4.35%
|
|
|
Current Opinion in Food Science
1 publication, 4.35%
|
|
|
Analytical Chemistry
1 publication, 4.35%
|
|
|
Metabolites
1 publication, 4.35%
|
|
|
Methods
1 publication, 4.35%
|
|
|
Russian Chemical Reviews
1 publication, 4.35%
|
|
|
1
2
3
|
Citations by publishers
|
2
4
6
8
10
12
14
16
|
|
|
Elsevier
15 publications, 65.22%
|
|
|
American Chemical Society (ACS)
2 publications, 8.7%
|
|
|
Future Medicine
1 publication, 4.35%
|
|
|
Springer Nature
1 publication, 4.35%
|
|
|
Wiley
1 publication, 4.35%
|
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 4.35%
|
|
|
Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 4.35%
|
|
|
2
4
6
8
10
12
14
16
|
- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2018,2019,2020,2021,2022,2023,2024],"ids":[0,0,0,0,0,0,0],"codes":[0,0,0,0,0,0,0],"imageUrls":["","","","","","",""],"datasets":[{"label":"Citations number","data":[2,2,7,3,4,3,2],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["8.7","8.7","30.43","13.04","17.39","13.04","8.7"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Journal of Chromatography A","Analytica Chimica Acta","Bioanalysis","Journal of Analysis and Testing","Journal of Pharmaceutical and Biomedical Analysis","Chinese Chemical Letters","Forensic Science International: Synergy","TrAC - Trends in Analytical Chemistry","Microchemical Journal","Acta Pharmaceutica Sinica B","Talanta","Journal of Neurochemistry","Chemical Research in Toxicology","Journal of Dairy Science","Current Opinion in Food Science","Analytical Chemistry","Metabolites","Methods","Russian Chemical Reviews"],"ids":[8970,19094,15951,15072,23304,20341,20964,434,15101,11233,4331,12,6125,10116,12599,18157,17024,16671,23802],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/AN2jnDN59NmKaS4CWLHAGstADL2DbnatQScsFA6C_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/9Mus3KG1Tkd7Bwaurt8H3RwWh0CxRlGoO6ng9UK1_medium.webp"],"datasets":[{"label":"","data":[3,2,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[13.04,8.7,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Elsevier","American Chemical Society (ACS)","Future Medicine","Springer Nature","Wiley","Multidisciplinary Digital Publishing Institute (MDPI)","Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii"],"ids":[17,40,6949,8,11,202,9422],"codes":[0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/AN2jnDN59NmKaS4CWLHAGstADL2DbnatQScsFA6C_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/9Mus3KG1Tkd7Bwaurt8H3RwWh0CxRlGoO6ng9UK1_medium.webp"],"datasets":[{"label":"","data":[15,2,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[65.22,8.7,4.35,4.35,4.35,4.35,4.35],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"yearsCitationsQuartiles":{"type":"bar","data":{"show":true,"labels":[2018,2019,2020,2021,2022,2023,2024],"ids":[],"codes":[],"imageUrls":[],"datasets":[{"label":"Q4","backgroundColor":"rgb(221,90,78)","data":[0,0,0,0,0,0,0],"percentage":["0","0","0","0","0","0","0"]},{"label":"Q3","backgroundColor":"rgb(251, 163,83)","data":[0,0,0,0,0,0,0],"percentage":["0","0","0","0","0","0","0"]},{"label":"Q2","backgroundColor":"rgb(232, 213, 89)","data":[1,0,0,0,1,1,0],"percentage":["4.35","0","0","0","4.35","4.35","0"]},{"label":"Q1","backgroundColor":"rgb(164, 207, 99)","data":[1,2,7,3,2,2,2],"percentage":["4.35","8.7","30.43","13.04","8.7","8.7","8.7"]},{"label":"Quartile not defined","backgroundColor":"#E5E7EB","data":[0,0,0,0,1,0,0],"percentage":["0","0","0","0","4.35","0","0"]}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations quartiles by SCImago per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"yearsCitationsQuartilesWs":{"type":"bar","data":{"show":true,"labels":[2018,2019,2020,2021,2022,2023,2024],"ids":[],"codes":[],"imageUrls":[],"datasets":[{"label":"Q4","backgroundColor":"rgb(221,90,78)","data":[0,0,0,0,0,0,0],"percentage":["0","0","0","0","0","0","0"]},{"label":"Q3","backgroundColor":"rgb(251, 163,83)","data":[1,0,0,0,0,0,0],"percentage":["4.35","0","0","0","0","0","0"]},{"label":"Q2","backgroundColor":"rgb(232, 213, 89)","data":[1,1,2,1,1,1,0],"percentage":["4.35","4.35","8.7","4.35","4.35","4.35","0"]},{"label":"Q1","backgroundColor":"rgb(164, 207, 99)","data":[0,1,3,2,2,2,2],"percentage":["0","4.35","13.04","8.7","8.7","8.7","8.7"]},{"label":"Quartile not defined","backgroundColor":"#E5E7EB","data":[0,0,2,0,1,0,0],"percentage":["0","0","8.7","0","4.35","0","0"]}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations quartiles by WoS per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Are you a researcher?
Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
Cite this
GOST |
RIS |
BibTex
Cite this
GOST
Copy
Guo H. et al. Twins labeling-liquid chromatography/mass spectrometry based metabolomics for absolute quantification of tryptophan and its key metabolites // Journal of Chromatography A. 2017. Vol. 1504. pp. 83-90.
GOST all authors (up to 50)
Copy
Guo H., Jiao Yu., Wang X., Lu T., Zhang Z., Xu F. Twins labeling-liquid chromatography/mass spectrometry based metabolomics for absolute quantification of tryptophan and its key metabolites // Journal of Chromatography A. 2017. Vol. 1504. pp. 83-90.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1016/j.chroma.2017.05.008
UR - https://doi.org/10.1016/j.chroma.2017.05.008
TI - Twins labeling-liquid chromatography/mass spectrometry based metabolomics for absolute quantification of tryptophan and its key metabolites
T2 - Journal of Chromatography A
AU - Guo, Huimin
AU - Jiao, Yu
AU - Wang, Xu
AU - Lu, Tao
AU - Zhang, Zunjian
AU - Xu, Fengguo
PY - 2017
DA - 2017/06/01 00:00:00
PB - Elsevier
SP - 83-90
VL - 1504
SN - 0021-9673
SN - 1873-3778
ER -
Cite this
BibTex
Copy
@article{2017_Guo,
author = {Huimin Guo and Yu Jiao and Xu Wang and Tao Lu and Zunjian Zhang and Fengguo Xu},
title = {Twins labeling-liquid chromatography/mass spectrometry based metabolomics for absolute quantification of tryptophan and its key metabolites},
journal = {Journal of Chromatography A},
year = {2017},
volume = {1504},
publisher = {Elsevier},
month = {jun},
url = {https://doi.org/10.1016/j.chroma.2017.05.008},
pages = {83--90},
doi = {10.1016/j.chroma.2017.05.008}
}