Colloids and Surfaces B: Biointerfaces, volume 219, pages 112834

Surface modification of fibroblasts with peroxiredoxin-1-loaded polymeric microparticles increases cell mobility, resistance to oxidative stress and collagen I production

Shen Ningfei ¹

Qi Xiaoli ¹

Bagrov Dmitry V ^{2, 3}

Krechetov Sergey P ⁴

Sharapov M. G. ⁵

Durymanov Mikhail ¹

Hide authors affiliations Show authors affiliations: 5 affiliations

Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Russia |

Faculty of Biology, Moscow State University, Moscow, Russia |

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia |

⁴

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Moscow, Russia |

⁵

Institute of Cell Biophysics of the Russian Academy of Sciences, Pushchino, Russia |

Publication type: Journal Article

Publication date: 2022-11-01

Elsevier

Journal: Colloids and Surfaces B: Biointerfaces

Quartile SCImago

Quartile WOS

Impact factor: 5.8

ISSN: 09277765, 18734367

DOI: 10.1016/j.colsurfb.2022.112834

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Physical and Theoretical Chemistry

General Medicine

Colloid and Surface Chemistry

Biotechnology

Surfaces and Interfaces

Abstract

Modification of the cell surface with artificial nano- and microparticles (also termed "cellular backpacks") containing biologically active payloads usually enables drug targeting via harnessing intrinsic cell tropism to the sites of injury. In some cases, using cells as delivery vehicles leads to improved pharmacokinetics due to extended circulation time of cell-immobilized formulations. Another rationale for particle attachment to cells is augmentation of desirable cellular functions and cell proliferation in response to release of the particle contents. In this study, we conjugated poly(lactic-co-glycolic acid) (PLGA) microparticles loaded with multifunctional antioxidant enzyme peroxiredoxin-1 (Prx1) to the surface of fibroblasts. The obtained microparticles were uniform in size and demonstrated sustained protein release. We found that the released Prx1 maintains its signaling activity resulting in macrophage activation, as indicated by TNFα upregulation and increase in ROS generation. Functionalization of fibroblasts with PLGA/Prx1 microparticles via EDC/sulfo-NHS coupling reaction did not affect cell viability but increased cell migratory properties and collagen I production. Moreover, PLGA/Prx1 backpacks increased resistance of fibroblasts to oxidative stress and attenuated cell senescence. In summary, we have developed a novel approach of fibroblast modification to augment their biological properties, which can be desirable for wound repair, cosmetic dermatology, and tissue engineering.

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International Journal of Molecular Sciences	International Journal of Molecular Sciences, 1, 100% International Journal of Molecular Sciences 1 publication, 100%
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Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 1, 100% Multidisciplinary Digital Publishing Institute (MDPI) 1 publication, 100%
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Shen N. et al. Surface modification of fibroblasts with peroxiredoxin-1-loaded polymeric microparticles increases cell mobility, resistance to oxidative stress and collagen I production // Colloids and Surfaces B: Biointerfaces. 2022. Vol. 219. p. 112834.

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Shen N., Qi X., Bagrov D. V., Krechetov S. P., Sharapov M. G., Durymanov M. Surface modification of fibroblasts with peroxiredoxin-1-loaded polymeric microparticles increases cell mobility, resistance to oxidative stress and collagen I production // Colloids and Surfaces B: Biointerfaces. 2022. Vol. 219. p. 112834.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1016/j.colsurfb.2022.112834

UR - https://doi.org/10.1016%2Fj.colsurfb.2022.112834

TI - Surface modification of fibroblasts with peroxiredoxin-1-loaded polymeric microparticles increases cell mobility, resistance to oxidative stress and collagen I production

T2 - Colloids and Surfaces B: Biointerfaces

AU - Shen, Ningfei

AU - Qi, Xiaoli

AU - Bagrov, Dmitry V

AU - Krechetov, Sergey P

AU - Sharapov, M. G.

AU - Durymanov, Mikhail

PY - 2022

DA - 2022/11/01 00:00:00

PB - Elsevier

SP - 112834

VL - 219

SN - 0927-7765

SN - 1873-4367

ER -

BibTex

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BibTex Copy

@article{2022_Shen,

author = {Ningfei Shen and Xiaoli Qi and Dmitry V Bagrov and Sergey P Krechetov and M. G. Sharapov and Mikhail Durymanov},

title = {Surface modification of fibroblasts with peroxiredoxin-1-loaded polymeric microparticles increases cell mobility, resistance to oxidative stress and collagen I production},

journal = {Colloids and Surfaces B: Biointerfaces},

year = {2022},

volume = {219},

publisher = {Elsevier},

month = {nov},

url = {https://doi.org/10.1016%2Fj.colsurfb.2022.112834},

pages = {112834},

doi = {10.1016/j.colsurfb.2022.112834}

}

Found error?

Publisher

Elsevier

Journal

Colloids and Surfaces B: Biointerfaces

Quartile SCImago

Quartile WOS

Impact factor

5.8

ISSN

09277765 (Print)
18734367 (Electronic)

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