Denatured BSA-Mediated Co-Assembly with Iron oxide Nanoparticles and Their Effect on Protein Fibrillation

Understanding the interaction between nanoparticles (NPs) and proteins provides a preliminary foundation for the further biomedical application of NPs. Herein, we disclosed that, unlike the natural bovine serum albumin (BSA) that interacts with iron oxide NPs (IONPs) to form a monolayer protein crown, the denatured BSA could co-assemble with IONPs to form stable protein coronal aggregates. Moreover, the effects of protein crown encapsulated IONPs on the anti-fibrotic ability was investigated. It was found that formation of protein crown on IONPs resulted in a weakened anti-fibrillation effect. Nevertheless, such adverse effect could be minimized by denatured BSA induced stable protein coronal aggregates.