Effective production of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) with Erwinia herbicola cells carrying a mutant transcriptional regulator TyrR
Takashi KOYANAGI
1
,
Takane Katayama
1
,
Hideyuki Suzuki
1
,
Hidetsugu Nakazawa
2
,
Kenzo Yokozeki
2
,
Hidehiko KUMAGAI
3
2
Fermentation and Biotechnology Laboratories, and AminoScience Laboratories, Ajinomoto Co., Inc., Kawasaki-shi, Kanagawa 210-8681, Japan
|
3
Research Institute of Agricultural Resources, Ishikawa Agricultural College, Nonoichi-cho, Ishikawa 921-8836, Japan
|
Publication type: Journal Article
Publication date: 2005-02-01
scimago Q2
wos Q2
SJR: 0.808
CiteScore: 8.5
Impact factor: 3.9
ISSN: 01681656, 18734863
PubMed ID:
15639092
General Medicine
Applied Microbiology and Biotechnology
Biotechnology
Bioengineering
Abstract
The enzymatic production of 3,4-dihydroxyphenyl-L-alanine (L-DOPA) using Erwinia herbicola cells involves the action of tyrosine phenol-lyase (Tpl, EC 4.1.99.2). Since Tpl is only synthesized under L-tyrosine-induced conditions, the addition of L-tyrosine to the medium is unavoidable when preparing cells (the enzyme source), but severely impedes the pure preparation of the final product L-DOPA. We circumvented this problem by using recombinant E. herbicola cells carrying a mutant transcriptional regulator TyrR, which is capable of activating the tpl promoter in the absence of L-tyrosine.
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GOST
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KOYANAGI T. et al. Effective production of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) with Erwinia herbicola cells carrying a mutant transcriptional regulator TyrR // Journal of Biotechnology. 2005. Vol. 115. No. 3. pp. 303-306.
GOST all authors (up to 50)
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KOYANAGI T., Katayama T., Suzuki H., Nakazawa H., Yokozeki K., KUMAGAI H. Effective production of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) with Erwinia herbicola cells carrying a mutant transcriptional regulator TyrR // Journal of Biotechnology. 2005. Vol. 115. No. 3. pp. 303-306.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1016/j.jbiotec.2004.08.016
UR - https://doi.org/10.1016/j.jbiotec.2004.08.016
TI - Effective production of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) with Erwinia herbicola cells carrying a mutant transcriptional regulator TyrR
T2 - Journal of Biotechnology
AU - KOYANAGI, Takashi
AU - Katayama, Takane
AU - Suzuki, Hideyuki
AU - Nakazawa, Hidetsugu
AU - Yokozeki, Kenzo
AU - KUMAGAI, Hidehiko
PY - 2005
DA - 2005/02/01
PB - Elsevier
SP - 303-306
IS - 3
VL - 115
PMID - 15639092
SN - 0168-1656
SN - 1873-4863
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2005_KOYANAGI,
author = {Takashi KOYANAGI and Takane Katayama and Hideyuki Suzuki and Hidetsugu Nakazawa and Kenzo Yokozeki and Hidehiko KUMAGAI},
title = {Effective production of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) with Erwinia herbicola cells carrying a mutant transcriptional regulator TyrR},
journal = {Journal of Biotechnology},
year = {2005},
volume = {115},
publisher = {Elsevier},
month = {feb},
url = {https://doi.org/10.1016/j.jbiotec.2004.08.016},
number = {3},
pages = {303--306},
doi = {10.1016/j.jbiotec.2004.08.016}
}
Cite this
MLA
Copy
KOYANAGI, Takashi, et al. “Effective production of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) with Erwinia herbicola cells carrying a mutant transcriptional regulator TyrR.” Journal of Biotechnology, vol. 115, no. 3, Feb. 2005, pp. 303-306. https://doi.org/10.1016/j.jbiotec.2004.08.016.