Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, volume 1164, pages 122511
A targeted ultra performance liquid chromatography – Tandem mass spectrometric assay for tyrosine and metabolites in urine and plasma: Application to the effects of antibiotics on mice
Marine P M Letertre
1
,
Antonis Myridakis
2
,
Luke Whiley
,
Stephane Camuzeaux
1, 3
,
Matthew M. Lewis
1, 3
,
Katie E. Chappell
1, 3
,
Annie Thaikkatil
1
,
M. Dumas
1
,
Jonathan Swann
1, 4
,
Ian D Wilson
1
Publication type: Journal Article
Publication date: 2021-02-01
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor: 3
ISSN: 15700232, 1873376X
Biochemistry
General Medicine
Cell Biology
Clinical Biochemistry
Analytical Chemistry
Abstract
• A rapid microbore RP-UHPLC-MS assay for tyrosine and 11 metabolites is described. • A “fit-for-purpose” approach was applied to method validation. • The assay was applied to mouse urine and plasma after feeding a high tyrosine diet. • Antibiotics revealed the contribution of the gut microbiota to tyrosine metabolism. Tyrosine plays a key role in mammalian biochemistry and defects in its metabolism ( e.g. , tyrosinemia, alkaptonuria etc.) have significant adverse consequences for those affected if left untreated. In addition, gut bacterially-derived p -cresol and its metabolites are of interest as a result of various effects on host xenobiotic metabolism. A fit-for-purpose quantitative ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) assay was developed to target and quantify tyrosine and eleven metabolites in urine and plasma. Dansylation, using dansyl chloride, was used to improve chromatographic and mass spectral properties for tyrosine and nine phenolic metabolites, with detection using positive electrospray ionisation (ESI). The sulfate and glucuronide conjugates of p -cresol, where the phenol group was blocked, were quantified intact, using negative ESI via polarity switching during the same run. Sample preparation for urine and plasma involved deproteinization by solvent precipitation (of acetonitrile:isopropyl alcohol (1:1 v/v)) followed by in situ dansylation in 96 well plates. To minimize sample and solvent usage, and maximize sensitivity, analysis was performed using microbore reversed-phase gradient UPLC on a C8 phase with a 7.5 min. cycle time. The coefficients of variation obtained were <15%, with lower limits of quantification ranging from 5 to 250 nM depending upon the analyte. The method was applied to plasma and urine samples obtained from mice placed on a high tyrosine diet with one subgroup of animals subsequently receiving antibiotics to suppress the gut microbiota. Whilst plasma profiles were largely unaffected by antibiotic treatment clear reductions in the amount of p -cresol sulfate and p -cresol glucuronide excreted in the urine were observed for these mice.
Top-30
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Microchemical Journal
1 publication, 11.11%
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Citations by publishers
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3
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Elsevier
3 publications, 33.33%
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Wiley
2 publications, 22.22%
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Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 22.22%
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Springer Nature
1 publication, 11.11%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 11.11%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Letertre M. P. M. et al. A targeted ultra performance liquid chromatography – Tandem mass spectrometric assay for tyrosine and metabolites in urine and plasma: Application to the effects of antibiotics on mice // Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2021. Vol. 1164. p. 122511.
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Letertre M. P. M., Myridakis A., Whiley L., Camuzeaux S., Lewis M. M., Chappell K. E., Thaikkatil A., Dumas M., Nicholson J. K., Swann J., Wilson I. D. A targeted ultra performance liquid chromatography – Tandem mass spectrometric assay for tyrosine and metabolites in urine and plasma: Application to the effects of antibiotics on mice // Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2021. Vol. 1164. p. 122511.
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TY - JOUR
DO - 10.1016/j.jchromb.2020.122511
UR - https://doi.org/10.1016/j.jchromb.2020.122511
TI - A targeted ultra performance liquid chromatography – Tandem mass spectrometric assay for tyrosine and metabolites in urine and plasma: Application to the effects of antibiotics on mice
T2 - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
AU - Letertre, Marine P M
AU - Myridakis, Antonis
AU - Whiley, Luke
AU - Camuzeaux, Stephane
AU - Lewis, Matthew M.
AU - Chappell, Katie E.
AU - Thaikkatil, Annie
AU - Dumas, M.
AU - Nicholson, Jeremy K
AU - Swann, Jonathan
AU - Wilson, Ian D
PY - 2021
DA - 2021/02/01 00:00:00
PB - Elsevier
SP - 122511
VL - 1164
SN - 1570-0232
SN - 1873-376X
ER -
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@article{2021_Letertre,
author = {Marine P M Letertre and Antonis Myridakis and Luke Whiley and Stephane Camuzeaux and Matthew M. Lewis and Katie E. Chappell and Annie Thaikkatil and M. Dumas and Jeremy K Nicholson and Jonathan Swann and Ian D Wilson},
title = {A targeted ultra performance liquid chromatography – Tandem mass spectrometric assay for tyrosine and metabolites in urine and plasma: Application to the effects of antibiotics on mice},
journal = {Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences},
year = {2021},
volume = {1164},
publisher = {Elsevier},
month = {feb},
url = {https://doi.org/10.1016/j.jchromb.2020.122511},
pages = {122511},
doi = {10.1016/j.jchromb.2020.122511}
}