Journal of Electroanalytical Chemistry, volume 856, pages 113655
Sensitive electrochemical aptasensor for detecting EpCAM with silica nanoparticles and quantum dots for signal amplification
Publication type: Journal Article
Publication date: 2020-01-01
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 4.5
ISSN: 15726657
General Chemical Engineering
Analytical Chemistry
Electrochemistry
Abstract
The epithelial cell adhesion molecule (EpCAM) is a known biomarker of circulating tumor cells that plays an important role in tumor metastasis. The detection of EpCAM is vital for personalized diagnosis and therapy but is challenging because of its extremely low concentration in peripheral blood. In our work, an electrochemical aptasensor has been developed to quantitatively detect EpCAM at low concentrations. A gold electrode modified with an aptamer can capture EpCAM, which can then be further recognized by a second aptamer acting as a signal reporter. To amplify the signal, a silica nanoparticle/CdSe complex has been designed to enhance the detection system. First, silica nanoparticles were synthesized to act as carriers for loading many CdSe quantum dots which are capable of binding with the second aptamer by biotin-streptavidin interactions. A molecule of EpCAM can bind with numerous CdSe quantum dots (QDs) to realize significant signal amplification. By stripping square wave voltammetry (SSWV), concentrations down to 10 aM EpCAM can be successfully detected and quantified based on the newly developed aptasensor. The detection linear range is from 10 aM to 100 pM. This principle can also be extended to other biomarkers when a suitable aptamer or antibody is available. This aptasensor is expected to be a potential tool for the diagnosis, therapeutic evaluation and personalized medical care of cancer patients.
Top-30
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2 publications, 14.29%
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1 publication, 7.14%
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1 publication, 7.14%
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1 publication, 7.14%
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1 publication, 7.14%
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1 publication, 7.14%
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1 publication, 7.14%
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1 publication, 7.14%
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1 publication, 7.14%
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Russian Chemical Reviews
1 publication, 7.14%
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1 publication, 7.14%
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1 publication, 7.14%
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Citations by publishers
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5
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Multidisciplinary Digital Publishing Institute (MDPI)
5 publications, 35.71%
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Elsevier
3 publications, 21.43%
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Bentham Science
1 publication, 7.14%
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American Chemical Society (ACS)
1 publication, 7.14%
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Springer Nature
1 publication, 7.14%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 7.14%
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Wiley
1 publication, 7.14%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Zhu L. et al. Sensitive electrochemical aptasensor for detecting EpCAM with silica nanoparticles and quantum dots for signal amplification // Journal of Electroanalytical Chemistry. 2020. Vol. 856. p. 113655.
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Zhu L., Yang B., Qian K., Liang Qiao L. Q., Liu Y., Liu B. Sensitive electrochemical aptasensor for detecting EpCAM with silica nanoparticles and quantum dots for signal amplification // Journal of Electroanalytical Chemistry. 2020. Vol. 856. p. 113655.
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TY - JOUR
DO - 10.1016/j.jelechem.2019.113655
UR - https://doi.org/10.1016/j.jelechem.2019.113655
TI - Sensitive electrochemical aptasensor for detecting EpCAM with silica nanoparticles and quantum dots for signal amplification
T2 - Journal of Electroanalytical Chemistry
AU - Zhu, Lina
AU - Yang, Beibei
AU - Qian, Kun
AU - Liang Qiao, Liang Qiao
AU - Liu, Yun
AU - Liu, Baohong
PY - 2020
DA - 2020/01/01 00:00:00
PB - Elsevier
SP - 113655
VL - 856
SN - 1572-6657
ER -
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@article{2020_Zhu,
author = {Lina Zhu and Beibei Yang and Kun Qian and Liang Qiao Liang Qiao and Yun Liu and Baohong Liu},
title = {Sensitive electrochemical aptasensor for detecting EpCAM with silica nanoparticles and quantum dots for signal amplification},
journal = {Journal of Electroanalytical Chemistry},
year = {2020},
volume = {856},
publisher = {Elsevier},
month = {jan},
url = {https://doi.org/10.1016/j.jelechem.2019.113655},
pages = {113655},
doi = {10.1016/j.jelechem.2019.113655}
}