Cell-derived Extracellular Matrix Proteins in Colloidal Microgel as a Self-Assembly Hydrogel for Regenerative Endodontics
Publication type: Journal Article
Publication date: 2022-04-01
scimago Q1
wos Q1
SJR: 1.229
CiteScore: 8.0
Impact factor: 3.6
ISSN: 00992399, 18783554
PubMed ID:
35077752
General Dentistry
Abstract
Abstract
Introduction
This study investigated a colloidal microgel for angiogenic and odontogenic differentiation of cells in the presence of cell-derived extracellular matrix (ECM) proteins using a 3-dimensional culture model.Methods
Viscoelastic properties of human dental pulp were determined to understand the native ECM environment. ECM proteins were extracted from dental pulp stem cell (DPSC) cultures, and MaxGel (Millipore Sigma, Burlington, MA) was used as a commercially available ECM protein. DPSCs were incubated in colloidal microgels in the presence of ECM proteins or gelatin methacryloyl (GelMA) as a bulk hydrogel (n = 9/group). The viability and odontogenic differentiation of DPSCs within hydrogels was determined using viability assays, mineralization staining, calcium and alkaline phosphatase assays, and quantitative polymerase chain reaction for odontogenic gene expression. Angiogenic properties of endothelial cells were determined using tubule formation assays and quantitative polymerase chain reaction to detect angiogenic gene expression.Results
Dental pulp had a higher elastic modulus than the viscous modulus, showing a solidlike response similar to hydrogels. DPSC-derived ECM showed higher collagen and GAG than MaxGel (P < .05). The viability of DPSCs was similar in colloidal microgels, whereas higher cell viability, calcium deposition, and alkaline phosphatase activity were observed in GelMA (P < .05). Colloidal microgels allowed tubule-like structures by endothelial cells, whereas no tubular formation was observed in GelMA. DPSC-derived ECM in colloidal microgel up-regulated odontogenic gene expression, whereas MaxGel up-regulated angiogenic gene expression (P < .05).Conclusions
Colloidal microgels allowed cellular organization that can improve penetration and nutritional supply in a full-length root canal system. The bioactivity of cell-derived ECM proteins can be modified depending on the external stimulus.Found
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17
Total citations:
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Citations from 2025:
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(17.65%)
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GOST
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Aksel H. et al. Cell-derived Extracellular Matrix Proteins in Colloidal Microgel as a Self-Assembly Hydrogel for Regenerative Endodontics // Journal of Endodontics. 2022. Vol. 48. No. 4. pp. 527-534.
GOST all authors (up to 50)
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Aksel H., Sarkar D., Meng Lin 蒙., Buck A., Huang G. J. Cell-derived Extracellular Matrix Proteins in Colloidal Microgel as a Self-Assembly Hydrogel for Regenerative Endodontics // Journal of Endodontics. 2022. Vol. 48. No. 4. pp. 527-534.
Cite this
RIS
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TY - JOUR
DO - 10.1016/j.joen.2022.01.011
UR - https://doi.org/10.1016/j.joen.2022.01.011
TI - Cell-derived Extracellular Matrix Proteins in Colloidal Microgel as a Self-Assembly Hydrogel for Regenerative Endodontics
T2 - Journal of Endodontics
AU - Aksel, Hacer
AU - Sarkar, Debanjan
AU - Meng Lin, 蒙林
AU - Buck, Andrew
AU - Huang, George J.
PY - 2022
DA - 2022/04/01
PB - Elsevier
SP - 527-534
IS - 4
VL - 48
PMID - 35077752
SN - 0099-2399
SN - 1878-3554
ER -
Cite this
BibTex (up to 50 authors)
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@article{2022_Aksel,
author = {Hacer Aksel and Debanjan Sarkar and 蒙林 Meng Lin and Andrew Buck and George J. Huang},
title = {Cell-derived Extracellular Matrix Proteins in Colloidal Microgel as a Self-Assembly Hydrogel for Regenerative Endodontics},
journal = {Journal of Endodontics},
year = {2022},
volume = {48},
publisher = {Elsevier},
month = {apr},
url = {https://doi.org/10.1016/j.joen.2022.01.011},
number = {4},
pages = {527--534},
doi = {10.1016/j.joen.2022.01.011}
}
Cite this
MLA
Copy
Aksel, Hacer, et al. “Cell-derived Extracellular Matrix Proteins in Colloidal Microgel as a Self-Assembly Hydrogel for Regenerative Endodontics.” Journal of Endodontics, vol. 48, no. 4, Apr. 2022, pp. 527-534. https://doi.org/10.1016/j.joen.2022.01.011.