Amperometric cholesterol biosensor based on cholesterol oxidase and Pt-Au/ MWNTs modified glassy carbon electrode
Publication type: Journal Article
Publication date: 2022-01-01
General Medicine
Abstract
The present work investigates the fabrication and performance studies of nanoscale metal cluster loaded Multi-Walled Carbon Nanotubes (MWNTs) based amperometric cholesterol biosensors. By a simple chemical reduction method Pt/ MWNTs, Au/MWNTs, and Pt-Au/MWNTs nanocomposites are prepared. Metal-decorated MWNTs are characterized by using X-ray diffraction analysis, scanning electron microscopy, and transmission electron microscopy (TEM). Amperometric biosensors are fabricated by depositing cholesterol oxidase (COx) enzyme on nanocrystalline metal/MWNTs modified GCE. Performance studies of these fabricated biosensors are conducted by using cyclic voltammetry and amperometry. The fabricated Au/MWNT based biosensor is best suited for the determination of cholesterol with a response time of 15 s, linear range from 2 µM to 1.4 mM, and limit of detection (S/N = 3) of 0.5 µM.
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12
Total citations:
12
Citations from 2024:
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(41.67%)
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Haritha V. S., Kumar S. R. S., Rakhi R. B. Amperometric cholesterol biosensor based on cholesterol oxidase and Pt-Au/ MWNTs modified glassy carbon electrode // Materials Today: Proceedings. 2022. Vol. 50. pp. 34-39.
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Haritha V. S., Kumar S. R. S., Rakhi R. B. Amperometric cholesterol biosensor based on cholesterol oxidase and Pt-Au/ MWNTs modified glassy carbon electrode // Materials Today: Proceedings. 2022. Vol. 50. pp. 34-39.
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TY - JOUR
DO - 10.1016/j.matpr.2021.03.128
UR - https://doi.org/10.1016/j.matpr.2021.03.128
TI - Amperometric cholesterol biosensor based on cholesterol oxidase and Pt-Au/ MWNTs modified glassy carbon electrode
T2 - Materials Today: Proceedings
AU - Haritha, V S
AU - Kumar, S R Sarath
AU - Rakhi, R. B.
PY - 2022
DA - 2022/01/01
PB - Elsevier
SP - 34-39
VL - 50
SN - 2214-7853
ER -
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@article{2022_Haritha,
author = {V S Haritha and S R Sarath Kumar and R. B. Rakhi},
title = {Amperometric cholesterol biosensor based on cholesterol oxidase and Pt-Au/ MWNTs modified glassy carbon electrode},
journal = {Materials Today: Proceedings},
year = {2022},
volume = {50},
publisher = {Elsevier},
month = {jan},
url = {https://doi.org/10.1016/j.matpr.2021.03.128},
pages = {34--39},
doi = {10.1016/j.matpr.2021.03.128}
}