Refolding of disulfide containing peptides in fusion with thioredoxin
Yulia A Logashina
1, 2
,
Yuliya V. Korolkova
3
,
Ekaterina E Maleeva
3
,
Dmitry I Osmakov
1, 2
,
Sergey V. Kozlov
3
,
Yaroslav A. Andreev
1, 2
Publication type: Journal Article
Publication date: 2020-03-01
scimago Q3
wos Q3
SJR: 0.305
CiteScore: 3.0
Impact factor: 1.7
ISSN: 09599436, 1364551X
General Chemistry
Abstract
A protocol for refolding of thioredoxin-fused cysteine-rich peptides via addition of oxidized/reduced glutathione reagent directly to unfolded soluble fused protein has been developed. This procedure allows one to skip the steps of inclusion bodies purification, denaturation/disulfide reduction as well as lyophilization before oxidative folding, and thus to improve the yield of cysteine-rich peptides in their production using E. coli expression system.
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GOST
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Logashina Y. A. et al. Refolding of disulfide containing peptides in fusion with thioredoxin // Mendeleev Communications. 2020. Vol. 30. No. 2. pp. 214-216.
GOST all authors (up to 50)
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Logashina Y. A., Korolkova Y. V., Maleeva E. E., Osmakov D. I., Kozlov S. V., A. Andreev Y. Refolding of disulfide containing peptides in fusion with thioredoxin // Mendeleev Communications. 2020. Vol. 30. No. 2. pp. 214-216.
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RIS
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TY - JOUR
DO - 10.1016/j.mencom.2020.03.028
UR - https://doi.org/10.1016/j.mencom.2020.03.028
TI - Refolding of disulfide containing peptides in fusion with thioredoxin
T2 - Mendeleev Communications
AU - Logashina, Yulia A
AU - Korolkova, Yuliya V.
AU - Maleeva, Ekaterina E
AU - Osmakov, Dmitry I
AU - Kozlov, Sergey V.
AU - A. Andreev, Yaroslav
PY - 2020
DA - 2020/03/01
PB - OOO Zhurnal "Mendeleevskie Soobshcheniya"
SP - 214-216
IS - 2
VL - 30
SN - 0959-9436
SN - 1364-551X
ER -
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@article{2020_Logashina,
author = {Yulia A Logashina and Yuliya V. Korolkova and Ekaterina E Maleeva and Dmitry I Osmakov and Sergey V. Kozlov and Yaroslav A. Andreev},
title = {Refolding of disulfide containing peptides in fusion with thioredoxin},
journal = {Mendeleev Communications},
year = {2020},
volume = {30},
publisher = {OOO Zhurnal "Mendeleevskie Soobshcheniya"},
month = {mar},
url = {https://doi.org/10.1016/j.mencom.2020.03.028},
number = {2},
pages = {214--216},
doi = {10.1016/j.mencom.2020.03.028}
}
Cite this
MLA
Copy
Logashina, Yulia A., et al. “Refolding of disulfide containing peptides in fusion with thioredoxin.” Mendeleev Communications, vol. 30, no. 2, Mar. 2020, pp. 214-216. https://doi.org/10.1016/j.mencom.2020.03.028.