Probing of microgel–enzyme films on graphite substrates by means of atomic force microscopy and amperometry
Publication type: Journal Article
Publication date: 2024-01-01
scimago Q3
wos Q3
SJR: 0.305
CiteScore: 3.0
Impact factor: 1.7
ISSN: 09599436, 1364551X
General Chemistry
Abstract
Microgel–enzyme coatings were fabricated onto graphite substrates (highly oriented pyrolytic graphite or graphite- based screen-printed electrodes) via two-stage sequential procedure, which consists in adsorption of a pH- and temperature-sensitive copolymer microgel, followed by electrostatic immobilization of glucose oxidase into the microgel film. The microgel–enzyme coatings were examined by means of atomic force microscopy showing remarkable coverage of graphite surface by the microgels and visualizing the immobilized enzyme. Finally, amperometry was applied to reveal the effect of the polymeric (microgel) matrix on temperature behavior of glucose oxidase in the microgel– enzyme coatings.
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Sigolaeva L. V., Efremova O. V., Pergushov D. V. Probing of microgel–enzyme films on graphite substrates by means of atomic force microscopy and amperometry // Mendeleev Communications. 2024. Vol. 34. No. 1. pp. 100-103.
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Sigolaeva L. V., Efremova O. V., Pergushov D. V. Probing of microgel–enzyme films on graphite substrates by means of atomic force microscopy and amperometry // Mendeleev Communications. 2024. Vol. 34. No. 1. pp. 100-103.
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TY - JOUR
DO - 10.1016/j.mencom.2024.01.030
UR - https://doi.org/10.1016/j.mencom.2024.01.030
TI - Probing of microgel–enzyme films on graphite substrates by means of atomic force microscopy and amperometry
T2 - Mendeleev Communications
AU - Sigolaeva, Larisa V.
AU - Efremova, Olga V
AU - Pergushov, Dmitry V.
PY - 2024
DA - 2024/01/01
PB - OOO Zhurnal "Mendeleevskie Soobshcheniya"
SP - 100-103
IS - 1
VL - 34
SN - 0959-9436
SN - 1364-551X
ER -
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@article{2024_Sigolaeva,
author = {Larisa V. Sigolaeva and Olga V Efremova and Dmitry V. Pergushov},
title = {Probing of microgel–enzyme films on graphite substrates by means of atomic force microscopy and amperometry},
journal = {Mendeleev Communications},
year = {2024},
volume = {34},
publisher = {OOO Zhurnal "Mendeleevskie Soobshcheniya"},
month = {jan},
url = {https://doi.org/10.1016/j.mencom.2024.01.030},
number = {1},
pages = {100--103},
doi = {10.1016/j.mencom.2024.01.030}
}
Cite this
MLA
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Sigolaeva, Larisa V., et al. “Probing of microgel–enzyme films on graphite substrates by means of atomic force microscopy and amperometry.” Mendeleev Communications, vol. 34, no. 1, Jan. 2024, pp. 100-103. https://doi.org/10.1016/j.mencom.2024.01.030.