Diagnosis of Helicobacter pylori bacterial infections using a voltammetric biosensor
2
Korea Ocean R&D Institute, P.O. Box 29, Ansan 425-600, South Korea
|
Publication type: Journal Article
Publication date: 2011-10-01
scimago Q3
wos Q3
SJR: 0.479
CiteScore: 3.9
Impact factor: 1.9
ISSN: 01677012, 18728359
PubMed ID:
21801757
Molecular Biology
Microbiology (medical)
Microbiology
Abstract
The voltammetric assay of Helicobacter pylori DNA was investigated using a bismuth-immobilized carbon nanotube electrode (BCNE). The analytical cyclic voltammetry (CV) peak potential was obtained at a 0.4V reduction scan, where the diagnostic optimum square-wave (SW) stripping working range was achieved at 0.72-7.92 μg/mL H. pylori DNA (11 points). A relative standard deviation of 1.68% (RSD, n=5) was obtained with 3.2 mg/mL H. pylori DNA using a 240 s accumulation time. Under optimum conditions, detection limit was 0.06 μg/mL. The developed sensors can be used for clinical application in the 15th doubted human gastric tissues, since the patient's peak current increased a hundred times more than the negative healthy tissue did. The sensing time obtained was only two minutes, and the process was simpler compared to common PCR amplification and electrophoresis photometric detection systems.
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28
Total citations:
28
Citations from 2024:
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(32.14%)
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GOST
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Ly S. Y., Yoo H., CHOA S. Diagnosis of Helicobacter pylori bacterial infections using a voltammetric biosensor // Journal of Microbiological Methods. 2011. Vol. 87. No. 1. pp. 44-48.
GOST all authors (up to 50)
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Ly S. Y., Yoo H., CHOA S. Diagnosis of Helicobacter pylori bacterial infections using a voltammetric biosensor // Journal of Microbiological Methods. 2011. Vol. 87. No. 1. pp. 44-48.
Cite this
RIS
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TY - JOUR
DO - 10.1016/j.mimet.2011.07.002
UR - https://doi.org/10.1016/j.mimet.2011.07.002
TI - Diagnosis of Helicobacter pylori bacterial infections using a voltammetric biosensor
T2 - Journal of Microbiological Methods
AU - Ly, Suw Young
AU - Yoo, Hai-Soo
AU - CHOA, SUNG-HOON
PY - 2011
DA - 2011/10/01
PB - Elsevier
SP - 44-48
IS - 1
VL - 87
PMID - 21801757
SN - 0167-7012
SN - 1872-8359
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2011_Ly,
author = {Suw Young Ly and Hai-Soo Yoo and SUNG-HOON CHOA},
title = {Diagnosis of Helicobacter pylori bacterial infections using a voltammetric biosensor},
journal = {Journal of Microbiological Methods},
year = {2011},
volume = {87},
publisher = {Elsevier},
month = {oct},
url = {https://doi.org/10.1016/j.mimet.2011.07.002},
number = {1},
pages = {44--48},
doi = {10.1016/j.mimet.2011.07.002}
}
Cite this
MLA
Copy
Ly, Suw Young, et al. “Diagnosis of Helicobacter pylori bacterial infections using a voltammetric biosensor.” Journal of Microbiological Methods, vol. 87, no. 1, Oct. 2011, pp. 44-48. https://doi.org/10.1016/j.mimet.2011.07.002.