Annales Pharmaceutiques Francaises, volume 78, issue 5, pages 398-407
Development and validation of RP-HPLC method for simultaneous estimation of docetaxel and ritonavir in PLGA nanoparticles.
Kumbhar P S
1
,
Diwate Sonali K
2, 3
,
Mali U G
1
,
Shinde T U
1
,
Disouza J I
1
,
Manjappa Arehalli S.
1
1
Tatyasaheb Kore College of Pharmacy, Warananagar, Panhala, 416113 Kolhapur, Maharashtra, India.
|
2
Tatyasaheb Kore College of Pharmacy, Warananagar, Panhala, 416113 Kolhapur, Maharashtra, India
|
3
Rajarshi Shahu Chatrapati Institute of Pharmacy, Kolhapur Karveer, 416002 Kolhapur, Maharashtra, India.
|
Publication type: Journal Article
Publication date: 2020-09-01
Journal:
Annales Pharmaceutiques Francaises
Quartile SCImago
Q3
Quartile WOS
—
Impact factor: 1.2
ISSN: 00034509
Pharmacology
Pharmaceutical Science
Abstract
Summary Objectives The main objective of the present study was to develop and validate simple, precise, sensitive and accurate RP-HPLC method for the simultaneous estimation of docetaxel (DTX) and ritonavir (RTV) in PLGA nanoparticles (PLGA-NPs). Methods The DTX and RTV co-loaded PLGA-NPs were developed by the nanoprecipitation technique. The RP-HPLC method was developed by using (Agilent Compact LC-1220) and Zorbax Eclipse plus C18 column (150 × 4.6 mm, 3.5 μm, Agilent). Finally, the developed method was validated according to the international conference on harmonization (ICH) guidelines. Results The chromatographic separations of DTX and RTV with good resolutions have been achieved by using the mobile phase Acetonitrile: Water (60:40 v/v) containing 0.1% v/v of orthophosphoric acid at a flow rate of 1.0 mL/min, injection volume of 25 μL, and at 239 nm wavelengths. The validated method found to be linear in the range of 0.001–100 μg/mL for DTX and RTV. Detection and quantification limits for DTX were found to be 0.7 and 2.31 μg/mL respectively and for RTV it is 0.3 and 2.87 μg/mL respectively. The % RSD was found to be less than 2% revealing the precision of the developed method. Besides, the recovery rate was observed close to 100% for both the drugs confirming the accuracy of the method. Minor alterations in the chromatographic conditions have revealed robustness and ruggedness of the developed method. Conclusion The developed analytical method is simple, precise, sensitive, and reproducible which can be used for the simultaneous estimation of DTX and RTV in the PLGA-NPs.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Kumbhar P. S. et al. Development and validation of RP-HPLC method for simultaneous estimation of docetaxel and ritonavir in PLGA nanoparticles. // Annales Pharmaceutiques Francaises. 2020. Vol. 78. No. 5. pp. 398-407.
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Kumbhar P. S., Diwate S. K., Mali U. G., Shinde T. U., Disouza J. I., Manjappa A. S. Development and validation of RP-HPLC method for simultaneous estimation of docetaxel and ritonavir in PLGA nanoparticles. // Annales Pharmaceutiques Francaises. 2020. Vol. 78. No. 5. pp. 398-407.
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TY - JOUR
DO - 10.1016/j.pharma.2020.07.004
UR - https://doi.org/10.1016%2Fj.pharma.2020.07.004
TI - Development and validation of RP-HPLC method for simultaneous estimation of docetaxel and ritonavir in PLGA nanoparticles.
T2 - Annales Pharmaceutiques Francaises
AU - Kumbhar, P S
AU - Diwate, Sonali K
AU - Mali, U G
AU - Shinde, T U
AU - Disouza, J I
AU - Manjappa, Arehalli S.
PY - 2020
DA - 2020/09/01 00:00:00
PB - Elsevier
SP - 398-407
IS - 5
VL - 78
SN - 0003-4509
ER -
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@article{2020_Kumbhar,
author = {P S Kumbhar and Sonali K Diwate and U G Mali and T U Shinde and J I Disouza and Arehalli S. Manjappa},
title = {Development and validation of RP-HPLC method for simultaneous estimation of docetaxel and ritonavir in PLGA nanoparticles.},
journal = {Annales Pharmaceutiques Francaises},
year = {2020},
volume = {78},
publisher = {Elsevier},
month = {sep},
url = {https://doi.org/10.1016%2Fj.pharma.2020.07.004},
number = {5},
pages = {398--407},
doi = {10.1016/j.pharma.2020.07.004}
}
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Kumbhar, P. S., et al. “Development and validation of RP-HPLC method for simultaneous estimation of docetaxel and ritonavir in PLGA nanoparticles..” Annales Pharmaceutiques Francaises, vol. 78, no. 5, Sep. 2020, pp. 398-407. https://doi.org/10.1016%2Fj.pharma.2020.07.004.