Plasmid, volume 77, pages 7-16
Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA38.
1
Biology Department, California State University Northridge, Northridge, CA 91330-8303, United States
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2
Ellington High School, Ellington, CT 06029, United States.
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3
Interdisciplinary Research Institute for the Sciences (IRIS), California State University Northridge, Northridge, CA 91330-8303, United States
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Publication type: Journal Article
Publication date: 2015-01-01
PubMed ID:
25450765
Molecular Biology
Abstract
We sequenced the complete 7118 bp circular plasmid pColE3-CA38 (pColE3) from Escherichia coli, located the previously identified colicin components together with two new ORFs that have homology to mobilization and transfer proteins, and found that pColE3 is highly similar to a plasmid present in enterohemorrhagic E. coli O111. We also found that unusual aspects of the plasmid include the inability to be completely digested with restriction endonucleases and asymmetric Phred DNA sequencing quality scores, with significantly lower scores in the forward direction relative to the colicin and immunity proteins consistent with plus (+) strand DNA. Comparing the A260 with picogreen double-stranded DNA (dsDNA) fluorescence and oligreen single-stranded DNA (ssDNA) fluorescence as well as metachromatic staining by acridine orange, we found that the undigested pColE3 DNA stains preferentially as ssDNA and that it coexists with dsDNA. We also identified ssDNA in pColE5 and pColE9 but not in pColE1. Colicin plasmids producing ssDNA may represent a new subclass of rolling-circle replication plasmids and add to the known similarities between colicins and filamentous phage.
Citations by journals
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
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Scientific Reports
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Scientific Reports
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Biotechnology and Bioengineering
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RNA Biology
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RNA Biology
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FEMS Microbiology Letters
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FEMS Microbiology Letters
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1
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Citations by publishers
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 20%
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Springer Nature
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Springer Nature
1 publication, 20%
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Wiley
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Wiley
1 publication, 20%
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Taylor & Francis
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Taylor & Francis
1 publication, 20%
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Oxford University Press
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Oxford University Press
1 publication, 20%
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Morales M. et al. Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA38. // Plasmid. 2015. Vol. 77. pp. 7-16.
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Morales M., Attai H., Troy K., Bermudes D. Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA38. // Plasmid. 2015. Vol. 77. pp. 7-16.
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TY - JOUR
DO - 10.1016/j.plasmid.2014.11.001
UR - https://doi.org/10.1016%2Fj.plasmid.2014.11.001
TI - Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA38.
T2 - Plasmid
AU - Morales, Magali
AU - Attai, Hedieh
AU - Troy, Kimberly
AU - Bermudes, David
PY - 2015
DA - 2015/01/01 00:00:00
PB - Elsevier
SP - 7-16
VL - 77
PMID - 25450765
SN - 0147-619X
SN - 1095-9890
ER -
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@article{2015_Morales,
author = {Magali Morales and Hedieh Attai and Kimberly Troy and David Bermudes},
title = {Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA38.},
journal = {Plasmid},
year = {2015},
volume = {77},
publisher = {Elsevier},
month = {jan},
url = {https://doi.org/10.1016%2Fj.plasmid.2014.11.001},
pages = {7--16},
doi = {10.1016/j.plasmid.2014.11.001}
}