Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, volume 238, pages 118433

Stability indicating spectrophotometric methods for quantitative determination of bromazepam and its degradation product

Hany W. Darwish ^{1, 2}

Nesma A Ali ³

Ibrahim A. Naguib ^{4, 5}

Mohamed R El Ghobashy ⁶

Abdullah M Al Hossaini ⁷

Nada S. Abdelwahab ⁸

Hide authors affiliations Show authors affiliations: 8 affiliations

Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., Cairo 11562, Egypt. |

Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia. |

Toxicology Laboratory, Forensic Medicine Authority, Justice Ministry,114 Bairam El Tounsy St., El Sayeda Zeinab, 11647 Cairo, Egypt |

⁴

Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, Al-Hawiah 21974, Taif, Saudi Arabia |

⁵

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St., 62514 Beni-Suef, Egypt |

⁶

Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., Cairo 11562, Egypt |

⁷

Department of Pharmaceutical Chemistry , College of Pharmacy -King Saud University , P. O. Box 2457, Riyadh, 11451, Saudi Arabia |

⁸

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St, 62514, Beni-Suef, Egypt |

Publication type: Journal Article

Publication date: 2020-09-01

Elsevier

Journal: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

Quartile SCImago

Quartile WOS

Impact factor: 4.4

ISSN: 13861425

DOI: 10.1016/j.saa.2020.118433

Copy DOI

Spectroscopy

Analytical Chemistry

Atomic and Molecular Physics, and Optics

Instrumentation

Abstract

Four simple, sensitive and selective stability indicating spectrophotometric methods are presented for quantitative determination of the benzodiazepine drug; bromazepam (BMZ) and one of its reported potential impurities and degradation product; 2-(2-amino-5-bromobenzoyl) pyridine (ABP) in methanol. Method A, is isoabsorptive point coupled with D0 method, where good linearity was obtained by measuring the absorbance of BMZ at 264 nm (Aiso) in the concentration range of 2–25 μg mL−1, and the absorbance of ABP at its λmax 396 nm in concentration range of 0.5–24 μg mL−1. Method B, is ratio subtraction; the absorbance was measured at 233 nm for BMZ using 20 μg mL−1 of ABP, while ABP was determined directly at its λmax 396 nm using methanol as a solvent. Method C, was based on measuring the total peak amplitude of the first derivative of the ratio spectra (DD1) of BMZ from 301 to 326 nm using 10 μg mL−1 of ABP as a divisor and determination of ABP at peak amplitude of 293 nm using 5 μg mL−1 of BMZ as a divisor. In method D, ratio difference method, good linearity was achieved for determination of BMZ and ABP by measuring the differences between the amplitudes of ratio spectra at 312 nm and 274 nm and differences between the amplitudes of ratio spectra at 274 nm and 312 nm, respectively. The stability of BMZ was investigated under different ICH recommended forced degradation conditions. The suggested methods were then successfully applied for determination of BMZ in its pharmaceutical formulations.

By date By citations

Top-30

Citations by journals

	1
Journal of Molecular Liquids	Journal of Molecular Liquids, 1, 12.5% Journal of Molecular Liquids 1 publication, 12.5%
Chemosensors	Chemosensors, 1, 12.5% Chemosensors 1 publication, 12.5%
Molecules	Molecules, 1, 12.5% Molecules 1 publication, 12.5%
Journal of Drug Delivery Science and Technology	Journal of Drug Delivery Science and Technology, 1, 12.5% Journal of Drug Delivery Science and Technology 1 publication, 12.5%
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 1, 12.5% Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy 1 publication, 12.5%
PLoS ONE	PLoS ONE, 1, 12.5% PLoS ONE 1 publication, 12.5%
Polymers	Polymers, 1, 12.5% Polymers 1 publication, 12.5%
Journal of Applied Spectroscopy	Journal of Applied Spectroscopy, 1, 12.5% Journal of Applied Spectroscopy 1 publication, 12.5%
	1

Citations by publishers

	1 2 3
Elsevier	Elsevier, 3, 37.5% Elsevier 3 publications, 37.5%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 3, 37.5% Multidisciplinary Digital Publishing Institute (MDPI) 3 publications, 37.5%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 1, 12.5% Public Library of Science (PLoS) 1 publication, 12.5%
Springer Nature	Springer Nature, 1, 12.5% Springer Nature 1 publication, 12.5%
	1 2 3

We do not take into account publications without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
Statistics recalculated weekly.

Are you a researcher?

Create a profile to get free access to personal recommendations for colleagues and new articles.

Metrics

Cite this

GOST |

Cite this

GOST Copy

Darwish H. W. et al. Stability indicating spectrophotometric methods for quantitative determination of bromazepam and its degradation product // Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2020. Vol. 238. p. 118433.

GOST all authors (up to 50) Copy

Darwish H. W., Ali N. A., Naguib I. A., El Ghobashy M. R., Al Hossaini A. M., Abdelwahab N. S. Stability indicating spectrophotometric methods for quantitative determination of bromazepam and its degradation product // Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2020. Vol. 238. p. 118433.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1016/j.saa.2020.118433

UR - https://linkinghub.elsevier.com/retrieve/pii/S138614252030411X

TI - Stability indicating spectrophotometric methods for quantitative determination of bromazepam and its degradation product

T2 - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

AU - Darwish, Hany W.

AU - Ali, Nesma A

AU - Naguib, Ibrahim A.

AU - El Ghobashy, Mohamed R

AU - Al Hossaini, Abdullah M

AU - Abdelwahab, Nada S.

PY - 2020

DA - 2020/09/01 00:00:00

PB - Elsevier

SP - 118433

VL - 238

SN - 1386-1425

ER -

BibTex

Cite this

BibTex Copy

@article{2020_Darwish,

author = {Hany W. Darwish and Nesma A Ali and Ibrahim A. Naguib and Mohamed R El Ghobashy and Abdullah M Al Hossaini and Nada S. Abdelwahab},

title = {Stability indicating spectrophotometric methods for quantitative determination of bromazepam and its degradation product},

journal = {Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy},

year = {2020},

volume = {238},

publisher = {Elsevier},

month = {sep},

url = {https://linkinghub.elsevier.com/retrieve/pii/S138614252030411X},

pages = {118433},

doi = {10.1016/j.saa.2020.118433}

}

Found error?

Publisher

Elsevier

Journal

Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

Quartile SCImago

Quartile WOS

Impact factor

4.4

ISSN

13861425 (Print)

Profiles

Naguib, Ibrahim A