volume 181 pages 575-583

A layer-by-layer assembled and carbon nanotubes/gold nanoparticles-based bienzyme biosensor for cholesterol detection

Publication typeJournal Article
Publication date2013-05-01
wos Q1
SJR
CiteScore
Impact factor7.7
ISSN09254005
Materials Chemistry
Metals and Alloys
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Electrical and Electronic Engineering
Instrumentation
Abstract
A bienzyme biosensor for the detection of cholesterol, based on functionalized carbon nanotubes (CNTs) was fabricated through layer-by-layer (LBL) assembly technique. Being wrapped with cationic poly(allylamine hydrochloride) (PAH), the nanocomposite, multiwalled carbon nanotubes (MCNTs) mixed with gold nanoparticles (GNPs) was water soluble and positively charged. Based on reliably electrostatic interaction, bienzyme biosensor was then fabricated by LBL assembly of the positively charged nanocomposite and the negatively charged enzymes, horseradish peroxidase (HRP) and cholesterol oxidase (ChOx). The fabricated process and the electrochemical behaviors of the biosensor were investigated by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Under the optimal conditions, the bienzyme biosensor obtained the linear range from 0.18 to 11 mM, with a detection limit of 0.02 mM. The proposed biosensor exhibited good sensitivity, stability, and controllability for the detection of cholesterol.
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GOST Copy
Cai X. et al. A layer-by-layer assembled and carbon nanotubes/gold nanoparticles-based bienzyme biosensor for cholesterol detection // Sensors and Actuators, B: Chemical. 2013. Vol. 181. pp. 575-583.
GOST all authors (up to 50) Copy
Cai X., Gao X., Lisha Wang L. W., Wu Q., Lin X. A layer-by-layer assembled and carbon nanotubes/gold nanoparticles-based bienzyme biosensor for cholesterol detection // Sensors and Actuators, B: Chemical. 2013. Vol. 181. pp. 575-583.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1016/j.snb.2013.02.050
UR - https://doi.org/10.1016/j.snb.2013.02.050
TI - A layer-by-layer assembled and carbon nanotubes/gold nanoparticles-based bienzyme biosensor for cholesterol detection
T2 - Sensors and Actuators, B: Chemical
AU - Cai, Xiao-Jun
AU - Gao, Xia
AU - Lisha Wang, Lisha Wang
AU - Wu, Qi
AU - Lin, Xianfu
PY - 2013
DA - 2013/05/01
PB - Elsevier
SP - 575-583
VL - 181
SN - 0925-4005
ER -
BibTex
Cite this
BibTex (up to 50 authors) Copy
@article{2013_Cai,
author = {Xiao-Jun Cai and Xia Gao and Lisha Wang Lisha Wang and Qi Wu and Xianfu Lin},
title = {A layer-by-layer assembled and carbon nanotubes/gold nanoparticles-based bienzyme biosensor for cholesterol detection},
journal = {Sensors and Actuators, B: Chemical},
year = {2013},
volume = {181},
publisher = {Elsevier},
month = {may},
url = {https://doi.org/10.1016/j.snb.2013.02.050},
pages = {575--583},
doi = {10.1016/j.snb.2013.02.050}
}