Quantitative butyrylcholinesterase activity detection by surface-enhanced Raman spectroscopy
Natalia L Nechaeva
1
,
Taisiya Prokopkina
2
,
G. F. Makhaeva
3
,
E. V. Rudakova
3
,
N. P. Boltneva
3
,
Christophor Dishovsky
4
,
Arkadiy Eremenko
1
,
Ilya N. Kurochkin
2
4
Military Medical Academy , Sofia , Bulgaria
|
Publication type: Journal Article
Publication date: 2018-04-01
wos Q1
SJR: —
CiteScore: —
Impact factor: 7.7
ISSN: 09254005
Materials Chemistry
Metals and Alloys
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Electrical and Electronic Engineering
Instrumentation
Abstract
The butyrylcholinesterase activity in human blood is an important marker of healthiness. Either level of this enzyme – increased or otherwise – can indicate disease or intoxication. The most popular technique to assess the cholinesterase activity is the detection of thiocholine, a product of enzymatic hydrolysis of butyrylthiocholine. In this work, Ag-paste was used as SERS-substrate and a new technique was created for thiocholine detection. As explained below, the designed technique facilitates also determination of butyrylcholinesterase (BChE) activity both in the buffer and in spike solution with human plasma. Ellman’s assay was used as a reference method, a good correlation between spectrophotometric detection and Raman detection was shown.
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47
Total citations:
47
Citations from 2024:
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(19.15%)
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Nechaeva N. L. et al. Quantitative butyrylcholinesterase activity detection by surface-enhanced Raman spectroscopy // Sensors and Actuators, B: Chemical. 2018. Vol. 259. pp. 75-82.
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Nechaeva N. L., Prokopkina T., Makhaeva G. F., Rudakova E. V., Boltneva N. P., Dishovsky C., Eremenko A., Kurochkin I. N. Quantitative butyrylcholinesterase activity detection by surface-enhanced Raman spectroscopy // Sensors and Actuators, B: Chemical. 2018. Vol. 259. pp. 75-82.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1016/j.snb.2017.11.174
UR - https://doi.org/10.1016/j.snb.2017.11.174
TI - Quantitative butyrylcholinesterase activity detection by surface-enhanced Raman spectroscopy
T2 - Sensors and Actuators, B: Chemical
AU - Nechaeva, Natalia L
AU - Prokopkina, Taisiya
AU - Makhaeva, G. F.
AU - Rudakova, E. V.
AU - Boltneva, N. P.
AU - Dishovsky, Christophor
AU - Eremenko, Arkadiy
AU - Kurochkin, Ilya N.
PY - 2018
DA - 2018/04/01
PB - Elsevier
SP - 75-82
VL - 259
SN - 0925-4005
ER -
Cite this
BibTex (up to 50 authors)
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@article{2018_Nechaeva,
author = {Natalia L Nechaeva and Taisiya Prokopkina and G. F. Makhaeva and E. V. Rudakova and N. P. Boltneva and Christophor Dishovsky and Arkadiy Eremenko and Ilya N. Kurochkin},
title = {Quantitative butyrylcholinesterase activity detection by surface-enhanced Raman spectroscopy},
journal = {Sensors and Actuators, B: Chemical},
year = {2018},
volume = {259},
publisher = {Elsevier},
month = {apr},
url = {https://doi.org/10.1016/j.snb.2017.11.174},
pages = {75--82},
doi = {10.1016/j.snb.2017.11.174}
}